A role of the Sox 9 gene in amphibian sex determination

Sox 9 基因在两栖动物性别决定中的作用

• 批准号: 12640652
• 负责人: NAKAMURA Masahisa
• 金额: $ 2.37万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640652/
• 关键词: Sox9; sex determination; sex differentiation; sex-reversal; testosterone; frog; isoforms; testis formation; sex differentiation; frog; Rana rugosa; testis; ovary

Sox is a family of SRY-related testis-determining genes. We have isolated two different mRNA isoforms of the frog Sox9 gene from adult frog testis cDNA library. One form (Sox9 α) encodes a 482-amino acid protein containing the HMG box, whereas the other (Sox9 β), which completely lacks the HMG box, is a N-terminal truncated 265-amino acid protein of Sox9 α. Sox9 α is 82 % similar to mouse, 86 % to chicken, and 77 % to trout Sox9s at the amino acid level. In the phylogenetic tree, frog Sox9 was clearly diverged from trout Sox9, and then from chicken and turtle Sox9s. The Sox9 expression was up-regulated in embryos after stage 16, and was seen in both differentiating testis and ovary. The size of Sox9 transcripts was determined to be 7.8 knt by Northern blot analysis. In addition, The expression of Sox9 α was dominant in the testis and brain among various tissues of adult frogs examined, and was considerably higher than that of Sox9 β. Sox9 is expressed at early stages of gonadal development in both sexes, but declined earlier in females. This is dissimilar to the pattern in birds and mammals, in which Sox9 expression is male-specific. In addition, Sox9 α and 9 β expressions were up-regulated in the gonad of female-to-male sex-reversed tadpoles. Taken together, the results suggest that the Sox9 is involved in testicular differentiation of amphibians. Since the Sox9 expression is found in both the testis and ovary of frogs at early stages of gonadal development, its role is probably different from that in birds and mammals.

SOX是一个与SRY相关的睾丸决定基因家族。我们从成蛙睾丸cDNA文库中分离到两种不同的青蛙Sox9基因的mRNA异构体。一种形式(Sox9α)编码一个含有HMG盒的482个氨基酸的蛋白质，而另一种形式(Sox9β)完全没有HMG盒，它是Sox9α的N端截短的265个氨基酸的蛋白。在氨基酸水平上，Sox9α与小鼠的同源性为82%，与鸡的同源性为86%，与鲑鱼的同源性为77%。在系统发育树上，青蛙Sox9明显与鲑鱼Sox9有明显的差异，然后又与鸡和甲鱼Sox9有明显的差异。Sox9在胚胎中的表达在16期以后上调，并在分化的睾丸和卵巢中都有表达。Northern印迹分析表明，Sox9转录本的大小为7.8knt。此外，SOX9α在成蛙睾丸和脑组织中的表达占主导地位，且显著高于SOX9β。SOX9在两性性腺发育的早期阶段都有表达，但在雌性性腺发育早期表达下降。这与鸟类和哺乳动物的模式不同，在鸟类和哺乳动物中，Sox9的表达是男性特有的。此外，SOX9α和9β在雌雄性反转蝌蚪的性腺中表达上调。综上所述，这些结果表明Sox9参与了两栖动物的睾丸分化。由于Sox9在青蛙性腺发育早期的睾丸和卵巢中都有表达，其作用可能与鸟类和哺乳动物不同。

项目成果

T.Nakajima et al.: "Two isoforms of FTZ-F1 messenger RNA : molecular cloning and their expression in the frog testis."Gene. 248. 203-212 (2000)

T.Nakajima 等人：“FTZ-F1 信使 RNA 的两种亚型：分子克隆及其在青蛙睾丸中的表达。”基因。

Takase.M.et al.: "FTZ-FIα is expressed in the developing go nad of frogs"Biochim. Biophys. Acta. 1494. 195-200 (2000)

Takase.M.et al.：“FTZ-FIα 在青蛙的发育中表达”Biochim. 1494. 195-200 (2000)。

Takase et al.: "Two Sox9 mRNA isoforms: isolation of cDNAs and their expression during gonadal development in the frog Rana rugosa"FEBS Letters. 466. 249-254 (2000)

Takase 等人：“两种 Sox9 mRNA 亚型：cDNA 的分离及其在青蛙性腺发育过程中的表达”FEBS Letters。

Nakajima et al.: "Two isoforms of FTZ-F1 messenger RNA: molecular cloning and their expression in the frog testis"GENE. 248. 203-212 (2000)

Nakajima 等人：“FTZ-F1 信使 RNA 的两种亚型：分子克隆及其在青蛙睾丸中的表达”GENE。

Kawano et al.: "Expression of steroidogenic factor-1 in frog embryo and developing gonad"Gen Comp.Endocrinol. 123. 13-22 (2001)

Kawano 等人：“青蛙胚胎和发育性腺中类固醇生成因子 1 的表达”Gen Comp.Endocrinol。