权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The sex-determining gene of amphibians having the XY-type sex-determining system differs from that of ones having the ZW-type sex-determining system

具有XY型性别决定系统的两栖动物的性别决定基因与具有ZW型性别决定系统的两栖动物的性别决定基因不同

基本信息

批准号：
17570053
负责人：
NAKAMURA Masahisa
金额：
$ 1.92万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570053/
关键词：
Sex-determining gene Karyotype Sex differentiation FISH RT-PCR cDNA Amphibian Rana rugosa ゲノムライブラリー

项目摘要

The aim of this research was to isolate the sex-determining gene in the frog Rana rugosa having two sex-determining systems (the XY and ZW types). We thought that the Dmrt1 gene was the best candidate for the sex-determining gene in R. rugosa having the ZW-type sex-determining system, since this gene was considered to be the sex-determining gene in chickens. However, FISH analysis revealed that Dmrt1 was an autosomal gene. The sex of the ZW type R. rugosa can be reversed from male to female by estrogens, whereas that of the XY type R. rugosa can be changed from female to male by androgens. However, the mechanism of the sex-reversal is largely unknown. To clarify the mechanism of the sex-reversal, we cloned and characterized 3 genes encoding sex steroid-synthesizing enzymes, i.e., CYP11A1, CYP17 and 3βHSD from the frog R. rugosa. RT-PCR analysis showed that the CYP17 expression was higher in male gonads during sex determination in R. rugosa, whereas CYP11A1 and 313-HSD showed no sexually dimorphic expression. When the sex was changed from female to male by injection of testosterone, the level of CYP17 transcripts increased in the sex-reversed gonad, whereas there was no change in the levels of CYP11A1 and 3β-HSD transcripts. In addition, in situ hybridization analysis revealed that CYP17 was expressed in somatic cells of the indifferent male gonad and in those of the testis. Positive signals of CYP17 were also produced in somatic cells of a female to male sex-reversed gonad (testis), but not in the ovary. Taken together, the results suggest that CYP17 is closely involved in testicular differentiation of the gonad in R. rugosa. CYP17 is the key-gene for female-to-male sex-reversal in R. rugosa.

本研究的目的是分离具有XY和ZW两种性别决定系统的皱纹蛙性别决定基因。我们认为Dmrt 1基因是决定红腹小球藻性别的最佳候选基因。rugosa具有ZW型性别决定系统，因为该基因被认为是鸡的性别决定基因。FISH分析表明Dmrt 1为常染色体基因。ZW型R的性别。Rugosa型的雌激素可使其从雄性变为雌性，而XY型的R.皱纹可以通过雄激素从雌性变成雄性。然而，性别逆转的机制在很大程度上是未知的。为了阐明性逆转的机制，我们克隆并鉴定了3个编码性类固醇合成酶的基因，蛙R.皱纹。RT-PCR分析表明，在雄蛙性别决定过程中，CYP 17在雄蛙性腺中的表达量较高。而CYP 11 A1和313-HSD则无性二态表达。注射睾酮后，性反转性腺中CYP 17的转录水平升高，而CYP 11 A1和3β-HSD的转录水平无明显变化。此外，原位杂交分析表明，CYP 17在无关雄性性腺和睾丸的体细胞中表达。CYP 17的阳性信号也产生于雌性到雄性的性反转性腺（睾丸）的体细胞中，但不在卵巢中。综上所述，这些结果表明，CYP 17密切参与了生殖腺的睾丸分化。皱纹。CYP 17基因是红腹小球藻性逆转的关键基因。皱纹。