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Forced Periodic and Chaotic Control of a Mixed Culture for Efficdient PHB Production

混合培养的强制周期性和混沌控制以实现高效 PHB 生产

基本信息

批准号：
12650791
负责人：
SHIMIZU Kazuyuki
金额：
$ 2.3万
依托单位：
KYUSHU INSTITUTE OF TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12650791/
关键词：
MIXED CULTURE PHB PERIODIC OPERATION DO CONTROL 水素細菌バイオリアクター周期制御

项目摘要

The unstructured mathematical model was developed for the mixed culture, where the metabolites produced by one microorganism is assimilated by the other microorganism.. For this, we specifically employed such model system that sugars such as glucose were converted to lactate by Lactobacillus delbrueckii and the lactate was converted in turn to poly β -hydroxybutyrate(PHB) by Ralstonia eutropha in one fermentor. Several batch and fed-batch culture experiments were conducted using each microorganism at different dissolved oxygen (DO) concentrations. Those experimental data were then fitted to the mathematical model, which can describe the dynamics of a mixed culture. Some of the model parameters were expressed as functions of DO concentrations, and some of the other model parameters were tuned based on the mixed culture experiments. The model developed describes the effects of such concentrations of glucose, lactate, DO, and NH_3 on the dynamic behavior of such concentrations as both microorganisms, glucose, lactate, and PHB. Optimal operating condition was then investigated using the model developed.It was found that the periodic change in DO concentration improved such performance as PHB yield, and it was verified by experiments. The effect of changing the DO concentration in chaotic manner on the performance improvement is not clear at this stage since very few experiments were conducted so far in this manner. This is the next topic to be pursued.

建立了混合培养过程的非结构化数学模型，其中一种微生物产生的代谢产物被另一种微生物同化。为此，我们特别采用了这样的模型系统，即在一个发酵罐中，糖如葡萄糖被德氏乳杆菌转化为乳酸盐，乳酸盐又被真养罗尔斯通氏菌转化为聚β -羟基丁酸酯（PHB）。使用每种微生物在不同的溶解氧（DO）浓度下进行几次分批和补料分批培养实验。然后将这些实验数据拟合到数学模型，该模型可以描述混合培养物的动力学。部分模型参数表示为溶解氧浓度的函数，部分模型参数根据混合培养实验进行调整。该模型描述了葡萄糖、乳酸、DO和NH_3等浓度对微生物、葡萄糖、乳酸和PHB等浓度动态行为的影响。利用所建立的模型对最佳操作条件进行了研究，发现DO浓度的周期性变化可以提高反应器的性能，并通过实验进行了验证。以混沌方式改变DO浓度对性能改善的影响在此阶段尚不清楚，因为迄今为止以这种方式进行的实验很少。这是下一个要探讨的主题。