Development of Integrated Lactic Acid Total Fermentation Processes

乳酸全发酵一体化工艺的开发

• 批准号: 06555249
• 负责人: SHIMIZU Kazuyuki
• 金额: $ 6.4万
• 依托单位: Kyushu Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06555249/
• 关键词: Lactic acid fermentation; Total system; Ceramic bioreactor; Optimisation; Extraction process; Ion-exchange resin; Cell-recycle; ろ過培養; 濾過培養

In the present research project, we developed an integrated lactic acid fermentation system which contains cell recycle fermentation system and the down-stream separation processes. In association with this system, we also investigated the related other research topics, Namely :(1) High cell density culture system was developed with ceramic membrane, and 183 g-dry cell weight/L could be attained with high cell viability.(2) The integrated fermentation system was developed which contains cell-recycle system and separation process. As the separation process, we considered to use extraction process using Alamin 336 as an extractant (oleyl alcohol was used as the solvent). It was shown to be quite important to find the pH values (4.2-4.5) for fermentation and extraction for the optimisation of total system.Furthermore, we considered to use ion-exchange resin as the separation unit. It was shown that high cell density culture with high substrate yield could be attained with this system.(3) An integrated on-line sensor system was developed to measure glucose and lactate on-line.(4) On-line optimising control system was investigated.(5) Mixed culture system using B.longum and P.freudenreichii was investigated to study the new activity of the reactor.(6) Respiratory chain and the energy yield were investigated for microorganisms.

在目前的研究项目中，我们开发了一种集成的乳酸发酵系统，其中包含细胞循环发酵系统和下游分离过程。与该系统相关，我们还研究了相关的其他研究课题，即：（1）采用陶瓷膜开发了高细胞密度培养系统，可以达到183克细胞干重/升，具有高细胞活力。（2）开发了包含细胞回收系统和分离过程的集成发酵系统。作为分离工艺，我们考虑采用以Alamin 336为萃取剂（以油醇为溶剂）的萃取工艺。结果表明，找到发酵和提取的pH值（4.2-4.5）对于整个系统的优化非常重要。此外，我们考虑使用离子交换树脂作为分离单元。结果表明，该系统可以实现高细胞密度培养和高底物产量。(3)开发了集成在线传感器系统，用于在线测量葡萄糖和乳酸。(4)研究了在线优化控制系统。(5)研究了长双歧杆菌和费氏丙酸杆菌的混合培养系统，以研究反应器的新活性。(6)研究了反应器的呼吸链和能量产率。 微生物。

项目成果

T.Kato,D.Yuguchi,K.Shimizu: "Performance Evaluation of lactic Acid Fermentation with Down-Stream Consideration" J.Chem.Eng.,Japan. (in preparation).

T.Kato、D.Yuguchi、K.Shimizu：“考虑下游因素的乳酸发酵性能评估”J.Chem.Eng.，日本。

K.Shimizu,K.Furuya,M.Taniguchi: "Optimal Operation Derived by Green's Theorem for the Cell-Recycle Filter Fermentation Focusing on the Efficient Use of the Medium" Biotechnol.Progress. 10. 258-262 (1994)

K.Shimizu、K.Furuya、M.Taniguchi：“由格林定理导出的细胞循环过滤发酵的优化操作，重点关注培养基的有效利用”生物技术进展。

K.Shimizu,K.Furuya,M.Taniguchi: "Optimal Operation Derived by Green's Theorem for the Cell-Recycle Filter Fermentation Focusing on the Efficient Use of the Medium" Biotechnology Progress. 10. 258-262 (1994)

K.Shimizu、K.Furuya、M.Taniguchi：“由格林定理导出的细胞循环过滤发酵的优化操作，重点关注培养基的有效利用”生物技术进展。

K.Ye,S.Jin,K.Shimzu: "Cell Recycle and Broth Reuse Fermentation with Cross-Flow Filtration and Ion-Exchange Resin" J.Chem.Tech.Biotechnol.66. 1-4 (1996)

K.Ye、S.Jin、K.Shimzu：“利用错流过滤和离子交换树脂进行细胞回收和肉汤再利用发酵”J.Chem.Tech.Biotechnol.66。

T.Suzuki: "Dense cell Retention Culture of L.lactis Using a Stirred ceramic membrane reactor" J.Fermentation and Bioengineering. (Submitted).

T.Suzuki：“使用搅拌陶瓷膜反应器进行乳酸乳球菌的密集细胞保留培养”J.Fermentation and Bioengineering。