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Development of a novel decay odor sensor which is working at normal temperature on the basis of electrochemiluminescence

基于电化学发光的新型常温腐烂气味传感器的研制

基本信息

批准号：
12650815
负责人：
EGASHIRA Naoyoshi
金额：
$ 2.24万
依托单位：
Hiroshima Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

1. Basic examination in aqueous solution.(1) Preparation of miniaturized cell: The cell was made where tris (bipyridine)ruthenium(II) complex was used as a luminescent. The electrochemiluminescence (ECL) measurement of amino acids and peptides showed that proline and peptides having an N-terminal proline residue gave strong responses. The use of the cell allowed us to determine analytes with a secondary or tertiary amine in the concentration range of 10^-6 M.(2) Preparation of the flow-through detector having protein-modified electrodes : The detector having a lysozyme-modified Au electrode showed the high selectivity for proline and the detector was applicable to a proline sensor.2. Preparation of decay-odor sensor.(1) Measurement conditions ; An electrolytic polymer gel was prepared by mixing tris(bipyridne) ruthenium complex, organic electrolyte, propylene carbonate, and an anionic polymer Nafion. A flow-through gas sensor was equipped with a Pt gauze working electrode, a Pt plate counter electrode, and a Ag wire reference electrode ; the gel was packed between the working and counter electrodes. The sensor strongly responded to trimethylamine gas and the stable work was attained with pulse electrolysis (El, +1.2V vs. Ag ; E2, +0.2V ; interval times of El and E2, each 0.1 Hz). The stability may be due to smaller heat evolution than that at direct electrolysis. A linear calibration curve was obtained in the concentration range from 10 to 310 ppb with low detection limit of 3 ppb (S/N = 3) ; the sensitivity was superior to that of the sense of smell in human.(2) Application to practical samples ; Seafood samples were allowed to stand in bags for 30 min and the gas evolved in bag was monitored by our sensor. The ECL response profile for squid was in good accordance with that obtained by the K value, showing that the sensor is also applicable to freshness sensor.

1.水溶液中的基本检查。(1)微型化电池的制备：在使用三（联吡啶）钌（II）络合物作为发光剂的情况下制备电池。氨基酸和肽的电化学发光（ECL）测量表明，脯氨酸和具有N-末端脯氨酸残基的肽给出了强烈的响应。使用该池，我们可以测定浓度范围为10^-6 M的仲胺或叔胺的分析物。(2)具有蛋白质修饰电极的流通式检测器的制备：具有溶菌酶修饰的Au电极的检测器显示出对脯氨酸的高选择性，并且该检测器适用于脯氨酸传感器.腐臭传感器的研制(1)测量条件;通过混合三（联吡啶）钌络合物、有机电解质、碳酸丙烯酯和阴离子聚合物Nafion来制备电解质聚合物凝胶。流通式气体传感器配备有Pt丝网工作电极、Pt板对电极和Ag丝参比电极;凝胶填充在工作电极和对电极之间。该传感器对三甲胺气体有很强的响应，并且在脉冲电解（E1，+1.2Vvs.Ag; E2，+0.2V ; E1和E2的间隔时间，每个0.1Hz）下获得稳定的工作。这种稳定性可能是由于比直接电解更小的放热。在10 ~ 310 ppb浓度范围内线性关系良好，检出限为3 ppb（S/N = 3），灵敏度上级优于人的嗅觉灵敏度。(2)应用于实际样品;海鲜样品被允许在袋中放置30分钟，并通过我们的传感器监测袋中的气体释放。鱿鱼的ECL响应曲线与由K值得到的响应曲线一致，表明该传感器也适用于鲜度传感器。