Development of a novel disposable film culture system using a high gas-permeable, thermal-stable and multilayer film

本研究的目的是（1）研究一种新型的OTP膜（OTP）是否符合“培养包”-岩棉系统（CP-RW）的要求，（2）然后开发一种由OTP制成的新型膜培养容器，即“Vitron”，其可以很好地适用于各种植物物种的离体克隆植株的发育;并且希望“Vitron”将在商业组织培养实验室中广泛使用。本研究中所用的外植体是从三个大花蕙兰品种1的大量离体芽中获得的具有三片叶子的芽。(1)、尾巨桉、甘薯、草莓和Cym.（二）、所用的底物是RW multiblock或Oasis无糖液体培养基和含糖琼脂和液体培养基。将膜培养系统置于25℃高CO_2浓度（3000 ppm）的培养室中进行培养。 ...更多信息 或非富集状态。在CP-RW中，观察到使用OTP的大花蕙兰幼苗的地上部和根部生长的促进作用，并且该值与PFA的值相当。两种膜培养系统的试管苗生长均比常规培养系统（琼脂培养基）的试管苗生长更快。在CP-RW培养基中，使用OTP和PFA培养的试管苗的光合速率高于瓶培养的试管苗。这些结果表明，OTP是一种良好的培养容器材料。与CP的实用模型“Miracle Pack”相比，使用OTP和PFA的“Vitron”中的桉树和甘薯试管苗的生长显著提高。草莓的生长和原球茎的增殖在“Vitron”系统中与OTP和PFA系统相当。这些结果显示了“Vitron”作为微繁殖模式培养容器的巨大潜力，因为该系统可以促进各种试管苗的体外生长的有利环境。此外，容器易于处理，从而减少了清洗的劳动力成本。通过应用这种容器系统，微繁殖将可用于许多重要的园艺、农业和林业植物的工业生产。少

The purposes of this study are (1) to investigate whether or not a novel OTP film (OTP), a super cheap film with similar characters as fluorocarbon polymer film (PFA), meets the requirements for the "Culture Pack"-Rockwool system (CP-RW), and (2) then to develop a new film culture vessel made of OTP, namely the "Vitron" that can be well-suited for the development of in vitro clonal plantlets of various plant species; and in a hope that the "Vitron" will be used widely in commercial tissue culture laboratories. The explants used in this study were shoots with three leaves obtained from a mass of in vitro shoots of three Cymbidium cultivars1 for exp. (1), and Eucalyptus uro-grandis, sweet potato, strawberry and a mass of Cym.protocorm-like bodies (PLBs) for exp. (2). The substrates used were RW multiblock or Oasis with sugar-free liquid medium, and sugar-containing agar and liquid medium. The film culture systems were placed in the culture room at 25℃ under high CO_2 enriched (3000 ppm) … More or non-enriched condition. Enhanced shoot and root growth of Cymbidium were observed in plantlets grown in CP-RW using OTP and the value was comparable to that of PFA. The growth of plantlets were enhanced more in both film culture systems than in a conventional system (flask with agar medium). Photosynthetic rate of the plantlets cultured in CP-RW using OTP and PFA was higher than that of the plantlets cultured in flask. These results indicate that OTP is a good material for developing culture vessel. The growth of Eucalyptus and sweet potato plantlets were drastically enhanced in the "Vitron" when compared with the "Miracle Pack" (the practical model of CP) using OTP and PFA. The growth of strawberry and the proliferation of PLB in the "Vitron" were comparable to that of OTP and PFA systems. These results shows great potential of the "Vitron" as a model culture vessel for micropropagation, since the system can facilitate a favorable environment for in vitro growth of various plantlet species. Moreover, the vessel is easily disposable thereby reducing labor cost for washing. By applying this vessel system, micropropagation would be accessible for industrial production of many important horticultural, agricultural and forestry plants. Less