Development of a novel disposable film culture system using a high gas-permeable, thermal-stable and multilayer film

本研究的目的是(1)研究一种新型的OTP薄膜(OTP)，这是一种与氟碳聚合物薄膜(PFA)具有相似特性的超廉价薄膜，是否满足“培养包”-岩棉系统(CP-RW)的要求；(2)开发一种由OTP制成的新型薄膜培养容器，即“Vitron”，它可以很好地适合各种植物的体外克隆植株的发育；并希望“Vitron”将在商业组织培养实验室中得到广泛应用。本研究所用的外植体是从三个兰花品种1的大量试管苗中获得的三片叶片的外植体。(1)、巨桉、红薯、草莓和大量原球茎(PLB)。(2)。所用基质为RW MULTLOCK或OASIS无糖液体培养基及含糖琼脂和液体培养基组分。将膜培养系统置于25℃、高CO_2浓度(3000ppm)…的培养室内更多或不丰富的情况。在CP-RW培养的试管苗中，用OTP促进了兰花的地上部和根部的生长，其效果与PFA相当。两种薄膜培养体系对试管苗生长的促进作用均大于常规培养体系(琼脂培养瓶)。采用OTP和PFA的CP-RW培养植株的光合作用速率均高于摇瓶培养的植株。这些结果表明，OTP是开发培养容器的良好材料。与采用OTP和PFA的“奇迹包装”(CP的实用模式)相比，“Vitron”显著促进了桉树和甘薯试管苗的生长。在“Vitron”培养体系中，草莓的生长和原球茎的增殖与OTP和PFA体系相当。这些结果表明，“Vitron”作为一种模式培养容器具有巨大的潜力，因为该系统可以为各种植物的体外生长创造良好的环境。此外，容器易于一次性使用，从而降低了洗涤的人力成本。通过应用该容器系统，许多重要的园艺、农业和林业植物的微繁殖将可用于工业化生产。较少

The purposes of this study are (1) to investigate whether or not a novel OTP film (OTP), a super cheap film with similar characters as fluorocarbon polymer film (PFA), meets the requirements for the "Culture Pack"-Rockwool system (CP-RW), and (2) then to develop a new film culture vessel made of OTP, namely the "Vitron" that can be well-suited for the development of in vitro clonal plantlets of various plant species; and in a hope that the "Vitron" will be used widely in commercial tissue culture laboratories. The explants used in this study were shoots with three leaves obtained from a mass of in vitro shoots of three Cymbidium cultivars1 for exp. (1), and Eucalyptus uro-grandis, sweet potato, strawberry and a mass of Cym.protocorm-like bodies (PLBs) for exp. (2). The substrates used were RW multiblock or Oasis with sugar-free liquid medium, and sugar-containing agar and liquid medium. The film culture systems were placed in the culture room at 25℃ under high CO_2 enriched (3000 ppm) … More or non-enriched condition. Enhanced shoot and root growth of Cymbidium were observed in plantlets grown in CP-RW using OTP and the value was comparable to that of PFA. The growth of plantlets were enhanced more in both film culture systems than in a conventional system (flask with agar medium). Photosynthetic rate of the plantlets cultured in CP-RW using OTP and PFA was higher than that of the plantlets cultured in flask. These results indicate that OTP is a good material for developing culture vessel. The growth of Eucalyptus and sweet potato plantlets were drastically enhanced in the "Vitron" when compared with the "Miracle Pack" (the practical model of CP) using OTP and PFA. The growth of strawberry and the proliferation of PLB in the "Vitron" were comparable to that of OTP and PFA systems. These results shows great potential of the "Vitron" as a model culture vessel for micropropagation, since the system can facilitate a favorable environment for in vitro growth of various plantlet species. Moreover, the vessel is easily disposable thereby reducing labor cost for washing. By applying this vessel system, micropropagation would be accessible for industrial production of many important horticultural, agricultural and forestry plants. Less