Development of a novel micropropagation system in Lilium longiflorum by film culture vessel

薄膜培养皿开发长花百合新型微繁殖系统

• 批准号: 04660035
• 负责人: TANAKA Michio
• 金额: $ 1.09万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660035/
• 关键词: Lilium longiflorum; Micropropagation; Film cullure vessel; Bulblet production

The bulblet production from Lilium longiflorum scales cultured in vitro was studied by using a novel disposable envelope-shaped culture vessel, the "Culture Bag" made of fluorocarbon polymer films (Neofron (〕SY.encircledR.〔) films). This paper dealt with the bulblet formation in scales cultured in vitro on modified Murashige and Skoog (MS) agarmedium (half macro-and micro-nutrients, 4.5% sucrose) in flask and in the liquid medium in the Culture Bag (90 x 40 mm) made of Neoflon FEP or PFA film of 12.5, 25, 50, and 100 mum thickness under light or dark condition. In both culture vessels, larger bulblets and higher number of bulblets per scale were obtained under light than dark. The number of bulblets per scale and fresh weight of bulbrets in any of the Culuture Bag were in fact higher when compared to those in flask. The number of foliage scales formed on bulbrets obtained in the Culuture Bag made of FEP film was higher than those in the Culuture Bag were in fact higher when compared to … More those in flask. The number of foliage scales formed on bulblets obtained in the Culture Bag made of FEP film was higher than those in the Culuture Bag made of PFA film. Further work showed that much larger bulblets were obtained in MS (full-strength nutrients, 4.5% sucrose) liquid medium in the Culture Bag made of PFA film compared to those on the agar medium in flask. An attempt was also made to develop the svale-up systems by using the Culuture Bag which is nine times larger (270 x 120 mm) than the smaller size and the 4-chambered Culuture Bag of larger size.the possible advatages to using the Culuture Bag system in the micropropagation of Lilium are as follows ; (1) bigger bulblets with very short foliage leaves and roots could be obtained than those in the conventional micropropagation, (2) these bulblets could be saved when inoculating explants to the large size Culuture Bag, and (4) the cost for the production of bulblets is expected to be much reduced by this novel system when vompared to those by the fermentor system and the tank culture system. Less

以麝香百合鳞片为外植体，采用新型一次性氟碳聚合物膜（Neofron（）SY. encircledR.（）电影）。本文研究了鳞片离体培养中小鳞茎的形成，在改良的Murashige和Skoog（MS）琼脂培养基（大量和微量营养物质各占一半，蔗糖4.5%）的烧瓶中和液体培养基中的培养袋（90 × 40 mm）由12.5，25，50，和100 μ m厚的Neoadenium FEP或PFA膜制成，在光照或黑暗条件下。在两种培养容器中，在光照下比黑暗下获得更大的小鳞茎和更高的每鳞片小鳞茎数。结果表明，每鳞片的小鳞茎数和小鳞茎鲜重均高于瓶内。在由FEP膜制成的培养袋中获得的鳞茎上形成的叶鳞的数量高于在培养袋中获得的鳞茎上形成的叶鳞的数量。 ...更多信息 那些在瓶子里。用FEP膜制作的培养袋培养的小鳞茎上形成鳞片的数量高于PFA膜制作的培养袋。进一步的研究表明，在MS（全营养液，4.5%蔗糖）液体培养基中，PFA膜制成的培养袋中的小鳞茎比三角瓶中的琼脂培养基中的小鳞茎大得多。还尝试通过使用大九倍的培养袋来开发svale-up系统（270 × 120 mm）的培养袋进行百合离体快繁，比小袋和大袋的4室培养袋快繁效果好。（1）与常规微繁殖相比，可获得较大的小鳞茎，其叶片和根都很短;（2）将外植体转移到大培养袋中可保存这些小鳞茎，（4）与发酵罐系统和罐式培养系统相比，这种新系统有望大大降低小鳞茎生产成本。少