Isolation of a cultivar-specific gene to soybean stunt virus

大豆矮化病毒品种特异性基因的分离

• 批准号: 12660037
• 负责人: MASUTA Chikara
• 金额: $ 1.6万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660037/
• 关键词: Pland virus; Soybean stunt virus; Resistance; ダイズ萎縮; SSV

Host resistance to plant viruses is specifically observed depending on host-virus combination. Using Cucumber mosaic virus(CMV) and its soybean starain (SSV), we analyzed the resistant phenotype that a virus does not systemicaily spread but it it is localized in the inoculated leaves in two host-virus combinations (host species-CMVs and soybean cultivars-SSVs). In the latter combination, we determined where the resistance mechanism operates and suggested existence of two complementary dominant host factors to control the sobybean cultivar resistance to SSV. Both in cucumber-CMV and in soybean-SSV combinations, the primary determinant for systemic infection is the domain including the 3a gene and depending on cultivars, an additional viral gene (the CP gene) was required in the cultivar resistance to SSV, the S region of RNA3 (mainly the 5' non-translated region) and the region including the entire 2b gene were together necessary for the systemic movement. It is therefore consistant with the observation that there are two host factors to affect the viral sysmtemic infection.

根据宿主病毒组合，特别观察到宿主对植物病毒的抗性。使用黄瓜镶嵌病毒（CMV）及其大豆星蛋白（SSV），我们分析了一种耐药表型，即病毒不会全身扩散，但它位于两个宿主病毒组合中的接种叶片中（宿主物种-CMVS和大豆型品种SSSVS）。在后一种组合中，我们确定了抗性机制在何处运作的位置，并建议存在两个互补的主要宿主因子来控制Sobybean品种对SSV的耐药性。在Cucumber-CMV和大豆-SSV组合中，全身感染的主要决定因素是包括3A基因的结构量，取决于品种，在品种耐种的SSV中需要附加的病毒基因（CP基因）（CP基因），RNA3的S SSV耐药性，RNA3的S SSV（主要是5'非跨性别区域）以及该区域包括该区域以及该区域以及该区域以及该区域。因此，观察到影响病毒系统感染的宿主因素有两个因素。

项目成果

Masuta, C.: "Development of clover yellow vein virus as an efficient, stable gene expression system for legume species."Plant Journal. 23. 539-546 (2000)

Masuta, C.：“将三叶草黄脉病毒开发为豆科植物高效、稳定的基因表达系统。”《植物杂志》。

Masuta, C. et al.: "Evolutionary characterization of two lily isolates of cucumber mosaic virus isolated in Japan and Korea"J. Gen. Plant Pathol.. (In press). (2002)

Masuta, C. 等人：“日本和韩国分离的两种黄瓜花叶病毒百合分离株的进化特征”J.

Masuta, C.etl al.: "Evolutionary characterization of two lily isolates of cucumber mosaic virus isolated in Japan and Korea"J.Gen.Plant Pathol.. (in press). (2002)

Masuta, C.etl al.：“在日本和韩国分离的黄瓜花叶病毒的两种百合分离株的进化特征”J.Gen.Plant Pathol..（正在出版）。

Masuta C. et al.: "Development of clover yellow vein virus as an efficient, stable gene expression system for legume species."Plant J.. 23. 539-546 (2000)

Masuta C. 等人：“将三叶草黄脉病毒开发为豆科植物高效、稳定的基因表达系统。”Plant J.. 23. 539-546 (2000)

Masuta et al.: "Development of clover yellow vein virus as an efficient, stable gene expression system for legume species"Plant J.. 23. 539-546 (2000)

Masuta 等人：“将三叶草黄脉病毒开发为豆科植物高效、稳定的基因表达系统”Plant J.. 23. 539-546 (2000)