Isolation of a cultivar-specific gene to soybean stunt virus

大豆矮化病毒品种特异性基因的分离

• 批准号: 12660037
• 负责人: MASUTA Chikara
• 金额: $ 1.6万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660037/
• 关键词: Pland virus; Soybean stunt virus; Resistance; ダイズ萎縮; SSV

Host resistance to plant viruses is specifically observed depending on host-virus combination. Using Cucumber mosaic virus(CMV) and its soybean starain (SSV), we analyzed the resistant phenotype that a virus does not systemicaily spread but it it is localized in the inoculated leaves in two host-virus combinations (host species-CMVs and soybean cultivars-SSVs). In the latter combination, we determined where the resistance mechanism operates and suggested existence of two complementary dominant host factors to control the sobybean cultivar resistance to SSV. Both in cucumber-CMV and in soybean-SSV combinations, the primary determinant for systemic infection is the domain including the 3a gene and depending on cultivars, an additional viral gene (the CP gene) was required in the cultivar resistance to SSV, the S region of RNA3 (mainly the 5' non-translated region) and the region including the entire 2b gene were together necessary for the systemic movement. It is therefore consistant with the observation that there are two host factors to affect the viral sysmtemic infection.

宿主对植物病毒的抗性取决于宿主-病毒组合。以黄瓜花叶病毒（CMV）及其大豆花叶病毒（SSV）为材料，分析了两种寄主-病毒组合（寄主-CMV和大豆品种-SSV）的抗病表型，即病毒不系统传播，但在接种叶片上定位。在后者的组合中，我们确定了抗性机制的运作，并建议存在两个互补的优势宿主因子来控制sobybean品种对SSV的抗性。在黄瓜-CMV和大豆-SSV组合中，系统感染的主要决定因素是包括3a基因的结构域，并且取决于品种，在品种对SSV的抗性中需要另外的病毒基因（CP基因），RNA 3的S区（主要是5'非翻译区）和包括整个2b基因的区域一起是系统运动所必需的。因此，这与观察到有两种宿主因素影响病毒的系统感染是一致的。

项目成果

Masuta, C.: "Development of clover yellow vein virus as an efficient, stable gene expression system for legume species."Plant Journal. 23. 539-546 (2000)

Masuta, C.：“将三叶草黄脉病毒开发为豆科植物高效、稳定的基因表达系统。”《植物杂志》。

Masuta, C. et al.: "Evolutionary characterization of two lily isolates of cucumber mosaic virus isolated in Japan and Korea"J. Gen. Plant Pathol.. (In press). (2002)

Masuta, C. 等人：“日本和韩国分离的两种黄瓜花叶病毒百合分离株的进化特征”J.

Masuta, C.etl al.: "Evolutionary characterization of two lily isolates of cucumber mosaic virus isolated in Japan and Korea"J.Gen.Plant Pathol.. (in press). (2002)

Masuta, C.etl al.：“在日本和韩国分离的黄瓜花叶病毒的两种百合分离株的进化特征”J.Gen.Plant Pathol..（正在出版）。

Masuta C. et al.: "Development of clover yellow vein virus as an efficient, stable gene expression system for legume species."Plant J.. 23. 539-546 (2000)

Masuta C. 等人：“将三叶草黄脉病毒开发为豆科植物高效、稳定的基因表达系统。”Plant J.. 23. 539-546 (2000)

Masuta et al.: "Development of clover yellow vein virus as an efficient, stable gene expression system for legume species"Plant J.. 23. 539-546 (2000)

Masuta 等人：“将三叶草黄脉病毒开发为豆科植物高效、稳定的基因表达系统”Plant J.. 23. 539-546 (2000)