Isolation of a resistance gene against potato virus Y from Solanacearum plants

从青枯植物中分离马铃薯 Y 病毒抗性基因

• 批准号: 09660355
• 负责人: MASUTA Chikara
• 金额: $ 2.11万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660355/
• 关键词: PVY; virus resistance; tobacco; cell-to-cell movement

Tobacco cultivar VAM is reported to have the recessive potyvirus resistance gene nu alpha. Varied levels of resistance were observed in VAM plants inoculated with Japanese potato virus Y (PVY) isolates. The VAM cultivar was highly resistant to most of the PVY isolates tested and tolerant to three necrotic strain isolates of PVY-T.Based on data obtained from tissue printing and press blotting, the resistance appeared to be mainly at the level of cell-to-cell movement. PVY replicated in VAM protoplasts, but the replication was 30% lower than in susceptible tobacco, suggesting that impairment of replication also contributes to resistance. To identify the viral gene product(s) involved in VAM resistance, we isolated spontaneous resistance-breaking mutants by passing vein-banding (0 strain) isolates several times through VAM plants. By comparing the amino acid sequences of the mutants and their original isolates, we identified a single amino acid substitution in the VPg domain that is correlated with VAM resistance breaking. Together, these results suggest that, in addition to its role in replication, VPg plays an important role in the cell-to-cell movement of PVY.

据报道，烟草品种VAM含有隐性抗马铃薯Y病毒基因nuα。在接种日本马铃薯Y病毒(PVY)分离物的VAM植株上观察到不同程度的抗性。该品种对供试的大部分PVY分离物高度抗性，对PVY-T的3个坏死株具有耐性。根据组织印迹和印迹分析，抗性主要发生在细胞间的运动水平上。PVY在VAM原生质体中复制，但复制比感病烟草低30%，表明复制障碍也与抗性有关。为了鉴定与VAM抗性有关的病毒基因产物(S)，我们通过在VAM植物上多次传代脉带(0株)的方法分离到自发的抗性打破突变体。通过比较突变株和其原始分离物的氨基酸序列，我们确定了VPG结构域上与VAM抗性打破相关的单一氨基酸替换。综上所述，这些结果表明，除了在复制中的作用外，VPG在PVY的细胞间移动中也起着重要的作用。

项目成果

Chikara Masuta: "Evolution of quadripartite hybrid virus by interspecific exchange and recombination between replicase components of two related tripartite RNA virases" Proc.Natl.Acad.Sci.USA. 95. 10487-10492 (1998)

Chikara Masuta：“通过两种相关三联 RNA 病毒的复制酶成分之间的种间交换和重组实现四联杂交病毒的进化”Proc.Natl.Acad.Sci.USA。