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Evaluaation of gene flow and reproduction cost in a tree population

树种群中基因流和繁殖成本的评估

基本信息

批准号：
12660138
负责人：
KAWAGUCHI Hideyuki
金额：
$ 2.24万
依托单位：
Shimane University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

The Japanese horse chestnut, Aesculus turbinata, is a tall deciduous tree species growing in cool temperate riparian forests in Japan. A. turbinata is andromonoecious and pollinated by mainly bumblebees. A. turbinata has large seeds which are dispersed by small mammals. A. turbinata occurs in riparian terrace and the lower part of slopes and often shows a linear-shaped distribution along a stream. Pollen flow, secondary seed dispersal, reproductive efforts, and genetic structure were analyzed in a linear-shaped population of A. turbinata along an upper narrow part of a stream. Six microsatellite makers were used for genetic analyses. Reproductive individuals of A. turbinata occurred about 1000m along the stream were sampled and analyzed as potential pollen donors. Seeds were collected from several individuals and their pollen donors were determined by microsatelite analyses. The number of pollen flow significantly decreased with increasing pollination distance. The amount of flowers and the timing of flowering in pollen donors were less effective on the number of pollen flow than the pollination distance. Fallen seeds were dispersed around mother tree by small mammals. Genetic similarity calculated from microsatellite analyses significantly decreased with increasing distance between individuals. The distance-dependent pollination and secondary seed dispersal around a mother tree may result in the spatial genetic structure in the A. turbinata population along the stream.

日本七叶树是生长在日本寒温带河岸森林中的高大落叶树种。大头黄蜂是雄雄同株的，主要由熊蜂授粉。涡虫有很大的种子，由小型哺乳动物传播。斑节藻见于河岸阶地和坡面下部，沿溪流呈线形分布。分析了沿河流上游狭窄部分的一个线形种群的花粉流动、次生种子传播、繁殖努力和遗传结构。六个微卫星标记被用于遗传分析。对沿溪流约1000m处发生的斑潜蝇生殖个体进行采样分析，作为潜在的供粉者。采集了几个个体的种子，通过微卫星分析确定了它们的供粉者。随着授粉距离的增加，花粉流数量显著减少。供粉者的花数和开花时间对花粉流数量的影响小于授粉距离。掉落的种子被小型哺乳动物散布在母树周围。根据微卫星分析计算的遗传相似度随着个体间距离的增加而显著降低。不同距离的授粉和次生种子在母树周围的传播可能导致了螺旋藻种群沿溪流的空间遗传结构。