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Gene expression on fructification of edible mushroom

食用菌结实期基因表达

基本信息

批准号：
12660153
负责人：
OHGA Shoji
金额：
$ 2.24万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Transcription of laccase and cellulase of Lentinula edodes was examined during fruit body development under different temperatures and moisture conditions on a sawdust-based substrate. Laccase transcript was maximal during the mycelial growth stage, and then declined rapidly at the fruiting stage. In contrast, cellulase transcript peaked at the veil-break stage during fruit body development. Transcriptions of these genes were triggered by microclimatic changes, specifically low temperature and osmotic pressure.Effect of electric impulse was investigated on fruit body formation of Lentinula edodes in the sawdust-based substrate. Electric impulse of 15 kV was directly charged to the substrate at 3 different fruiting phases, 90-, 130- and 170-day cultures, respectively. Ergosterol contents and water conditions of the substrate were examined at the 3 phases. The electric impulse was effective to fruit body formation of 4 strains in 3 different fruiting types, which were for year-round cult … More ivation, for cultivation in spring and autumn, and for cultivation in winter. The effect of the electric impulse was more prominent as culture age proceeds.Effect of electric impulse was investigated on fruit body formation of Lentinula edodes in the sawdust-based substrate. Electric impulse of 15 kV was directly charged to the substrate at 3 different fruiting phases, 90-, 130- and 170-day cultures, respectively. Ergosterol contents and water conditions of the substrate were examined at the 3 phases. The electric impulse was effective to fruit body formation of 4 strains in 3 different fruiting types, which were for year-round cultivation, for cultivation in spring and autumn, and for cultivation in winter. The effect of the electric impulse was more prominent as culture age proceeds.Ribonucleic acid (RNA-M) and 5' nucleotide mixture (RNA-Nt) were added to the mycelial colony (shiro) of Tricholoma matsutake prior to fruiting stage. Forty g ribonucleic acid derivatives was dissolved in 4,000 ml distilled water, and sprayed directly to 4 m^2 surface ground of the pine forest. Glucosamine ergosterol and phospholipid were useful indicators to determine the fungal biomass in the fine roots. These indicator components in the fine roots increased after RNA-M and RNA-Nt addition, and then revealed obviously higher levels compare with non-addition control plots. Addition of these compounds resulted to mycelial biomass increment judging from 3 indicator responses. Fruit body formation occurred around 30 days after addition of RNA-M and RNA-Nt. Total amount of fruit body was 230 g (9 fruit bodies) (RNA-M) and 210 g (7 fruit bodies) (RNA-Nt) during 14 days observation period. In contrast, yield of control plots were 0-60 g (0-2 fruit bodies) in the experimental area. Yield and number of T. matsutake resulted increment with the addition of nucleic acid related compounds. Less

在不同温度和湿度条件下，在锯末基质上检查香菇子实体发育过程中漆酶和纤维素酶的转录。漆酶转录本在菌丝生长阶段达到最大，然后在子实体阶段迅速下降。相反，纤维素酶转录在子实体发育过程中的破纱阶段达到顶峰。这些基因的转录是由微气候变化触发的，特别是低温和渗透压。研究了电脉冲对木屑基质中香菇子实体形成的影响。 15 kV 的电脉冲在 3 个不同的结果期（分别为 90 天、130 天和 170 天的培养物）直接对基质充电。在三个阶段检查底物的麦角甾醇含量和水条件。电脉冲对全年栽培、春秋栽培、冬季栽培3个不同果型的4个品系的子实体形成有效。随着培养龄的增长，电脉冲的影响更加明显。研究了电脉冲对木屑基质中香菇子实体形成的影响。 15 kV 的电脉冲在 3 个不同的结果期（分别为 90 天、130 天和 170 天的培养物）直接对基质充电。在三个阶段检查底物的麦角甾醇含量和水条件。电脉冲对全年栽培、春秋栽培、冬季栽培3个不同果型的4个品系的子实体形成有效。随着培养年龄的增长，电脉冲的效果更加突出。在出菇阶段之前，将核糖核酸（RNA-M）和5'核苷酸混合物（RNA-Nt）添加到松茸菌丝体菌落（shiro）中。将40克核糖核酸衍生物溶解在4,000毫升蒸馏水中，并直接喷洒到松林的4 m^2地面地面上。葡萄糖胺麦角甾醇和磷脂是测定细根中真菌生物量的有用指标。添加RNA-M和RNA-Nt后细根中的这些指标成分增加，并且与未添加对照区相比显着升高。从 3 个指标响应来看，添加这些化合物导致菌丝体生物量增加。添加RNA-M和RNA-Nt后约30天发生子实体形成。在14天的观察期内，子实体总量为230克（9个子实体）（RNA-M）和210克（7个子实体）（RNA-Nt）。相比之下，试验区对照地块的产量为0-60克（0-2个子实体）。随着核酸相关化合物的添加，松茸的产量和数量增加。较少的