Decomposition of low degradable chelating agent by marine bacteria bioremediation of marine environment

海洋细菌分解低降解螯合剂海洋环境生物修复

• 批准号: 12660166
• 负责人: IMADA Chiaki
• 金额: $ 2.24万
• 依托单位: Tokyo University of Fisheries
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660166/
• 关键词: EDTA; Chelating agent; marine bacteria; Pseudoalteromonas; Vibrio; Pseudomonas; Alteromonas; Flavobacterium; Acinetobacter

Chelating agent such as ethylenediaminetetraacetic acid (EDTA)-decomposing bacteria were first isolated from marine environment and characterized. The rate of degradation of EDTA was measured by ion chromatography as ferric chelate. Out of 885 isolates from various sea areas, 8 strains isolated from various sea areas were found to decompose it. Among them, strain H6-2-4 had the highest ability to decompose it. The rate of degradation was approximately 30 % in seawater medium containing 1000 ppm of Fe-EDTA. The experiment using the intact cells revealed that the strain also had an ability to decompose various metal-EDTA chelates such as Ca, Cu and Mg. EDTA was converted into iminodiacetic acid, which was further cleaved into glycine in the second pathway. The strain was a Gram-negative motile rod with polar flagella and required seawater for its growth, which indicated that it was marine in origin. The strain was identified as Pseudoalteromonas atlantica based on the DNA-DNA hybridization with the type culture.

螯合剂如乙二胺四乙酸（EDTA）分解细菌首次从海洋环境中分离和表征。EDTA的降解速率通过离子色谱法测定为铁螯合物。从885株不同海域分离的菌株中，筛选出8株能降解该物质的菌株，其中菌株H6-2-4的降解能力最强，在含1000 ppm Fe-EDTA的海水培养基中，降解率约为30%。使用完整细胞的实验表明，该菌株还具有分解各种金属-EDTA螯合物如Ca、Cu和Mg的能力。EDTA转化为亚氨基二乙酸，其在第二途径中进一步裂解为甘氨酸。该菌株为革兰氏阴性杆菌，具有极鞭毛，需要海水才能生长，表明该菌株为海洋来源。通过与典型培养物的DNA-DNA杂交，鉴定该菌株为大西洋交替假单胞菌（Pseudoalteromonasatlantica）。