Decomposition of low degradable chelating agent by marine bacteria bioremediation of marine environment

首次从海洋环境中分离得到乙二胺四乙酸(EDTA)等螯合剂降解菌并对其进行了表征。以铁络合物形式，用离子色谱法测定EDTA的降解率。在来自不同海域的885个菌株中，有8个菌株对其有分解作用。其中，菌株H6-2-4的降解能力最强。在含1000ppm Fe-EDTA的海水介质中，降解率约为30%。利用完整细胞进行的实验表明，该菌株还具有分解各种金属-EDTA络合物的能力，如钙、铜和镁。EDTA转化为亚氨基二乙酸，亚氨基二乙酸在第二条途径中进一步裂解成甘氨酸。该菌株为革兰氏阴性杆菌，具有极地鞭毛，需要海水才能生长，表明该菌株起源于海洋。经DNA-DNA杂交鉴定，该菌株为大西洋假交替单胞菌。

Chelating agent such as ethylenediaminetetraacetic acid (EDTA)-decomposing bacteria were first isolated from marine environment and characterized. The rate of degradation of EDTA was measured by ion chromatography as ferric chelate. Out of 885 isolates from various sea areas, 8 strains isolated from various sea areas were found to decompose it. Among them, strain H6-2-4 had the highest ability to decompose it. The rate of degradation was approximately 30 % in seawater medium containing 1000 ppm of Fe-EDTA. The experiment using the intact cells revealed that the strain also had an ability to decompose various metal-EDTA chelates such as Ca, Cu and Mg. EDTA was converted into iminodiacetic acid, which was further cleaved into glycine in the second pathway. The strain was a Gram-negative motile rod with polar flagella and required seawater for its growth, which indicated that it was marine in origin. The strain was identified as Pseudoalteromonas atlantica based on the DNA-DNA hybridization with the type culture.