Decomposition of low degradable chelating agent by marine bacteria bioremediation of marine environment

首次从海洋环境中分离到螯合剂乙二胺四乙酸（EDTA）分解菌，并对其进行了表征。用离子色谱法测定了EDTA作为铁螯合物的降解速率。从各海域分离的885株菌株中，发现有8株菌株能分解该菌。其中菌株H6-2-4的分解能力最强。在含1000ppm Fe-EDTA的海水介质中，降解率约为30%。利用完整细胞进行的实验表明，该菌株还具有分解各种金属- edta螯合物（如Ca， Cu和Mg）的能力。EDTA转化为亚氨基二乙酸，在第二途径中进一步裂解为甘氨酸。该菌株为革兰氏阴性运动杆，具有极性鞭毛，生长需要海水，表明其起源于海洋。通过与型培养的DNA-DNA杂交鉴定该菌株为大西洋假异单胞菌（Pseudoalteromonas atlantica）。

Chelating agent such as ethylenediaminetetraacetic acid (EDTA)-decomposing bacteria were first isolated from marine environment and characterized. The rate of degradation of EDTA was measured by ion chromatography as ferric chelate. Out of 885 isolates from various sea areas, 8 strains isolated from various sea areas were found to decompose it. Among them, strain H6-2-4 had the highest ability to decompose it. The rate of degradation was approximately 30 % in seawater medium containing 1000 ppm of Fe-EDTA. The experiment using the intact cells revealed that the strain also had an ability to decompose various metal-EDTA chelates such as Ca, Cu and Mg. EDTA was converted into iminodiacetic acid, which was further cleaved into glycine in the second pathway. The strain was a Gram-negative motile rod with polar flagella and required seawater for its growth, which indicated that it was marine in origin. The strain was identified as Pseudoalteromonas atlantica based on the DNA-DNA hybridization with the type culture.