Studies on protective function of glycoprotein in fish blood cell membrane(glycophorin) toward fish pathogen.

Glycophorin是哺乳动物和鸟类血液中红细胞膜中的一种转膜半胱氨酸蛋白质。鲤鱼（塞浦路斯·卡皮奥）血液被抽出来，准备了鲤鱼红色细胞膜。膜蛋白质和糖基酚素是由SDS-聚丙烯酰胺凝胶电泳测试的。Cumassie蓝色条纹图案的卷曲红细胞薄膜与彩虹鳟鱼和人类相似。突出的膜蛋白质，波段3，是为人类红细胞膜设计的，被检测到如光谱素、ankyrin和actin。与PAS斯坦一起，在Carp红细胞膜中检测到糖蛋白。Carp Glycophorin是用Lis-phenol方法从膜中提取出来的，这个准备显示了较弱的N型血液活动。碳水化合物中的唾液酸被薄层色谱法和其他方法确定为NGNA。从氨基酸分析的结果来看，该组合与人类糖素相似。However，碳水化合物中寡氯酮的组成与人类寡核苷酸的差异 ... More Saccharide。Carp Glycophorin和各种讲座的相互作用显示了Carp Glycophorin的寡链组件与O-链接（Gal·GalNAc·Ser/Thr）、α Glc和Fuc。在β-elimination之后，释放了从glycophorin收集的寡链，并通过使用阴离子交换柱色谱法（HPLC）分离出两个片段。这些片段是氢化的，随后是用脉冲安培测量检测器（HPAEC-PAD）分析高性能阴离子交换色谱法。HPAEC-PAD的结果也提出了关于寡氯硫化合物成分是Gal、Glc和Fuc的结论。此外，这一结果也得到了核磁共振光谱的加强，这些寡藻电荷的结构在没有GC-MS分析数据的情况下无法确定。我们正在准备用于GC-MS分析的毫克级非饱和寡核苷酸，从电子显微镜观察，发现了附着到革兰阴性细菌的片状的细胞壁，并将其转化为革兰阳性细菌的细胞壁。这些结果提出了一个关于碳水化合物糖素具有一个保护性功能的鱼病原体。Less（低）

Glycophorin is a transmembrane sialoglycoprotein in the red cell membrane from mammalian and avian blood. Carp (Cyprinus carpio) blood was drawn, and prepared the carp red cell membrane. Membrane proteins and glycophorin were examined by SDS- polyacrylamide gel electrophoresis. The Coomassie blue stain pattern of carp red cell membrane was similar to that of rainbow trout and human. The prominent membrane protein, band 3 which designated for human red cell membrane, was detected as well as spectrin, ankyrin and actin. With PAS stain, glycoprotein was detected in the carp red cell membrane. Carp glycophorin was extracted from the membrane by LIS-phenol method, and this preparation showed weak N blood type activity. Sialic acid in carp glycophorin was determined NGNA by thin layer chromatography and other methods. From the result of amino acid analysis, composition was similar to human glycophorin. However, composition of oligosaccharide in carp glycophorin was differing from human oligo … More saccharide. The interaction of carp glycophorin and various lectins showed that the components of carp glycophorin's oligossacharides were O-linked (Gal・GalNAc・Ser/Thr), α Glc and Fuc. After β-elimination, released oligosaccharides from glycophorin were collected, and separated two fractions by using anion exchange column chromatography (HPLC). These fractions were hydrolyzed, following by analysis of high performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD). The results from HPAEC-PAD also suggested that the components of oligosaccharides were Gal, Glc and Fuc. Although this results being strengthen by NMR spectra, the structure of these oligosaccharides were not determined without data from GC-MS analysis. We are going to preparation of mg-order desalted oligosaccharides for GC-MS analysis.From the observation by electron microscope, it seemed that carp glycophorin molecules attached to the flagellum of gram-negative bacterium and to the cell wall of gram-positive bacterium. These results suggested that carp glycophorin has a protective function toward fish pathogen. Less