Effect of the exposure to circularly polarized magnetic field on the metabolism of pteridine and biogenic amines

圆极化磁场暴露对蝶啶和生物胺代谢的影响

• 批准号: 12831001
• 负责人: MASADA Masahiro
• 金额: $ 2.37万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12831001/
• 关键词: magnetic field; pteridine; tetrahydrobiopterin; neopterin; mouse; 回転円磁界; 生体アミン

Tetrahydrobiopterin(BH4) is a putative cofactor for the hydroxylation of aromatic amino acids and its level has been indicated to regulate the initial and rate limiting enzymes in the pathway for biosynthesis of cateholamines and indolamines. Other pteridines, such as neopterin(NP) and pterin(AHP), are postulated to correlate with immunity and aging. It is reported that melatonin level is lowered by the exposure to circularly polarized magnetic field. The objective if this study is to determine the alteration of each pteridine level in mouse tissues under the exposure to circularly polarized magnetic field.Five male mice per group, five week-old at the start, were used and exposed continuously to 0.5 mT circularly polarized magnetic field in period of 1,3 and 6 weeks. The sham-animal was exposed to stray field about 0.14 μ T. Mouse tissues were obtained after decapitation at the end of each exposure period and stored frozen at -80 ℃ until assayed. Data obtained from both groups were co … More mpared with that cage control group. Statistical significance of different between three groups was performed by Students's t-testThere were no difference if body weights among three groups. The level of NP and AHP in tissues from exposed group were significantly increased from other groups. BH4 content was significantly decreased in the exposed group, although total biopterin level was not different among three groups. No differences of GTP cyclohydrolase I and pyruvoyltetrahydropterin synthase activities were recognized among three groups.These results suggests that BH4 biosynthesis is not affected by the exposure to magnetic field. The speculation leads to that the exposure to magnetic field may affect the reduction of quinonoid-dihydrobiopterin to BH4. The decreaqse of BH4 level may result in a lowering of amounts of ccatechoamines and indolamines. Further studies are carrying in the alteration of the neurotransmittance level under exposure to circularly polarized magnetic field and the effect of linearly polarized magnetic field on pteridine levels. Less

四氢生物蝶呤（BH 4）是芳香族氨基酸羟基化的辅助因子，其水平可调节cateholamine和indolamine生物合成途径中的起始酶和限速酶。其他蝶啶，如新蝶呤（NP）和蝶呤（AHP），被认为与免疫和衰老有关。据报道，褪黑激素水平降低暴露于圆极化磁场。为研究圆极化磁场对小鼠组织中蝶啶含量的影响，选用5周龄雄性小鼠，每组5只，连续暴露于0.5mT的圆极化磁场中1、3、6周。假手术组动物暴露于0.14 μ T的杂散场。在每个暴露期结束时断头后获得小鼠组织，并在-80 ℃下冷冻储存直至测定。从两组中获得的数据是一致的。 ...更多信息 与笼子对照组进行比较。三组之间的差异用Students’s t检验进行统计学意义。三组之间的体重无差异。暴露组组织中NP和AHP水平较其他组显著升高。暴露组的BH 4含量显著降低，而总生物蝶呤水平在三组之间没有差异。三组间的GTP环化水解酶I和乙酰四氢蝶呤合成酶活性无明显差异，表明磁场对BH 4的生物合成没有影响。推测磁场暴露可能影响类醌-二氢生物蝶呤还原为BH_4。BH_4水平的降低可能导致儿茶素胺和吲哚胺含量的降低。圆极化磁场对神经递质水平的影响以及线极化磁场对蝶啶水平的影响正在进一步研究中。少