UCP induction on the inner cell and mitochondrial membrances of Xenopus oocyte injected with gene transcripts from brown adipocytes of cold acclimated rat

注射冷驯化大鼠棕色脂肪细胞基因转录本的非洲爪蟾卵母细胞内细胞和线粒体膜上的 UCP 诱导

基本信息

  • 批准号:
    12670069
  • 负责人:
  • 金额:
    $ 2.43万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

The RNA transcripts were purified from the brown adipocytes of cold and warm acclimated rats by the acid guanidium isothiocyanate phenol chloroform (AGPC) method. Whole cell clamp recordings of the oocyte which were injected with the RNA transcript purified from cold acclimated rats, revealed a voltage dependent large conductance, while no conductance was recorded on the oocyte injected with the transcript from warm acclimated rat. Light and electron microscopic observations showed the appearance of newly synthesized inner membrane near the surface of oocytes which were injected with RNA transcripts purified from cold acclimated rat brown adipocytes. Immuno-gold electron microscopy using anti UCP1 polyclonal antibody revealed the localization of immuno-gold particles on the inner membrane of mitochondria like structure of the newly synthesized membrane in oocytes injected with the cold acclimated rat transcript.
用酸性异硫氰酸胍酚氯仿(AGPC)法从冷、热适应大鼠棕色脂肪细胞中提取RNA转录物。将冷适应大鼠的RNA转录物注射到卵母细胞中,用全细胞钳记录显示了电压依赖性的大电导,而将热适应大鼠的RNA转录物注射到卵母细胞中则没有电导记录。光镜和电镜观察显示,新合成的内膜表面附近的卵母细胞注射从冷驯化大鼠棕色脂肪细胞纯化的RNA转录本的外观。免疫金电子显微镜使用抗UCP1多克隆抗体显示定位的免疫金颗粒的线粒体样结构的新合成的膜在卵母细胞注射冷驯化大鼠转录的内膜。

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nakagaki I, Tsai YL, Sasaki S, Hori S, Kondo H: "Effects of cold acclimation for mitochondrial ion transport activity in brown adiocytes"Jpn J Physiol. 51(Suppl). S271 (2001)
Nakagaki I、Tsai YL、Sasaki S、Hori S、Kondo H:“冷驯化对棕色脂肪细胞线粒体离子转运活性的影响”Jpn J Physiol。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Tsai YL, Sasaki S, Nakagaki I, Tsujita J, Hori S, Hori, K: "Ion transport and morphological changes of mitochondria in brown adipocytes of warm- and cold-acclimated obese zucker rats."Jpn J Physiol. 51. 531-537 (2001)
Tsai YL、Sasaki S、Nakagaki I、Tsujita J、Hori S、Hori、K:“热适应和冷适应的肥胖 zucker 大鼠棕色脂肪细胞中线粒体的离子转运和形态变化。”Jpn J Physiol。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Nakagaki I, Sasaki S.Hori S.KondoH.: "Ca2+ and electrolyte mobilization following agonist application to the pancreatic B cell line HIT."Pflugers Archiv,. 440. 828-834 (2000)
Nakagaki I、Sasaki S.Hori S.KondoH.:“将激动剂应用于胰腺 B 细胞系 HIT 后的 Ca2 和电解质动员。”Pflugers Archiv,。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Sasaki S, Tsai YL, Nakagaki I.Yahata T, Kanoh N, Hori S, Kuroshima A: "Changes of ion environment during UCP induction in rat brown adipocytes."Jpn J Physiol. 49(Supple). S231 (1999)
Sasaki S、Tsai YL、Nakagaki I.Yahata T、Kanoh N、Hori S、Kuroshima A:“大鼠棕色脂肪细胞 UCP 诱导过程中离子环境的变化。”Jpn J Physiol。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Nakagaki I, Tsai YL, Sasaki S, Hori S, Kondo H: "Effects of cold acclimation for mitochondria! ion transport activity in brown adipocytes."Jpn J Physiol. 51(Supple). S271 (2001)
Nakagaki I、Tsai YL、Sasaki S、Hori S、Kondo H:“冷适应对线粒体的影响!棕色脂肪细胞中的离子转运活性。”Jpn J Physiol。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

SASAKI Sadao其他文献

SASAKI Sadao的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('SASAKI Sadao', 18)}}的其他基金

Electrophysiological and electron microprobe studies of ion transport in cold acclimated brown adipocyte.
冷适应棕色脂肪细胞中离子传输的电生理学和电子微探针研究。
  • 批准号:
    08670092
  • 财政年份:
    1996
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Relating gene expression to protein regulation for increased cardiac function in zebrafish (Danio rerio) during cold acclimation
将基因表达与冷驯化期间斑马鱼(Danio rerio)心脏功能增强的蛋白质调节联系起来
  • 批准号:
    580823-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
Characterization and Functional Assessment of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Protein and Receptor Expression at the Stellate and Celiac Ganglia in Male and Female Mice Following Cold Acclimation
冷适应后雄性和雌性小鼠星状神经节和腹腔神经节垂体腺苷酸环化酶激活多肽 (PACAP) 蛋白和受体表达的表征和功能评估
  • 批准号:
    429000
  • 财政年份:
    2019
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Studentship Programs
Stimulating nonshivering thermogenesis using cold acclimation in humans
利用冷适应刺激人类非颤抖产热
  • 批准号:
    525106-2018
  • 财政年份:
    2018
  • 资助金额:
    $ 2.43万
  • 项目类别:
    University Undergraduate Student Research Awards
The role of ion-motive ATPas regulation in the cold acclimation of drosophila
离子动力ATP酶调节在果蝇冷驯化中的作用
  • 批准号:
    514419-2017
  • 财政年份:
    2017
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
Temperature and light signals in plant cold acclimation
植物冷驯化中的温度和光信号
  • 批准号:
    17H03961
  • 财政年份:
    2017
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Stimulating nonshivering thermogenesis in humans by cold acclimation
通过冷适应刺激人类非颤抖产热
  • 批准号:
    511341-2017
  • 财政年份:
    2017
  • 资助金额:
    $ 2.43万
  • 项目类别:
    University Undergraduate Student Research Awards
The role of OEP16 and OEP40 in cold acclimation (B05)
OEP16和OEP40在冷驯化中的作用(B05)
  • 批准号:
    317552149
  • 财政年份:
    2016
  • 资助金额:
    $ 2.43万
  • 项目类别:
    CRC/Transregios
Coordination of vacuolar proton-pumps during cold acclimation
冷驯化过程中液泡质子泵的协调
  • 批准号:
    317306136
  • 财政年份:
    2016
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Research Grants
Gene expression, inositol metabolism and cold acclimation in Drosophila.
果蝇的基因表达、肌醇代谢和冷驯化。
  • 批准号:
    NE/P000592/1
  • 财政年份:
    2016
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Research Grant
Molecular physiology of tissue circuit for cold acclimation
冷驯化组织回路的分子生理学
  • 批准号:
    15H04404
  • 财政年份:
    2015
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了