Ionic mechanisms for spontaneously beating in monkey sino-atrial nodal cells

猴窦房结细胞自发搏动的离子机制

• 批准号: 12670093
• 负责人: SATOH Hiroyasu
• 金额: $ 2.24万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670093/
• 关键词: Sino-atrial nodal cells; Monkey heart; Automaticity; Pace-maker currents; Spontaneously beating; Ionic channels; Pharmacological receptors; 洞房結節細胞; 受容体

The mechanism for automaticity of sino-atrial nodal cells has been investigated using rabbit, guinea pig and rat. However, monkey sino-atrial nodal cells mostly resemble to human being have never been examined, because of difficult experimental techniques and economical supports. We have already established a technique for an isolation of sino-atrila nodal cells of monkey by many repeated trials. Whole-cell and current clamp patch-clamp experiments were carried out. We found that the Ionic mechanisms for the initiation of spontaneously beating monkey sino-atrial nodal cells are different from other species. In monkey sino-atrial nodal cells, the hyper polarization-activated inward current (I_f) was not markedly activated even at strong pulses. This means that the I_f current may be not always contributed to the normal spontaneously beating.To elucidate the ionic mechanisms for the automaticity in monkey sino-atrial nodal eelIs plays an important role for clinically treatment and prevention of heart diseases such as arrhythmias, cardiac failure and sudden death.

用兔、豚鼠和大鼠研究了窦房结细胞自律化的机制。然而，由于实验技术的困难和经济上的支持，大多数与人类相似的猴窦房结细胞从未被检测过。经过多次反复试验，我们已经建立了一种分离猴窦房结细胞的技术。进行了全细胞膜片钳实验和电流钳实验。我们发现，自发搏动的猴窦房结细胞启动的离子机制与其他物种不同。在猴窦房结细胞中，超极化激活的内向电流(If)即使在强脉冲时也不明显被激活。阐明猴窦房结自发搏动的离子机制，对临床治疗和预防心律失常、心力衰竭、猝死等心脏疾病具有重要意义。

项目成果

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Tohno Y.：“人类二尖瓣随衰老而发生的成分变化。Biol”trac。

Satoh H.: "Electropharmacology of Giukgo biloba extract and the constituents in guinea pig ventrieular cavdiomyoaytes"Jpn.J.Pharmacol.. 88. 259 (2002)

Satoh H.：“Giukgo biloba 提取物的电药理学和豚鼠心室腔肌细胞中的成分”Jpn.J.Pharmacol.. 88. 259 (2002)

Satoh H.: "Electropharmacology of Ginkgo biloba extract and the constituents in gunea pig ventricular cardiomyocytes"Jpn. J. Pharmacol. 88. 259 (2002)

Satoh H.：“银杏叶提取物的电药理学和豚鼠心室心肌细胞成分”Jpn。

Satoh H.: "Comparetive actions of cibenzoline and disopyramide on Ikr and Iks currents in rat sine-atrial node cells"Eur.J.Pharmacol.. 407. 123-129 (2000)

Satoh H.：“西苯唑啉和丙吡胺对大鼠正弦心房结细胞 Ikr 和 Iks 电流的比较作用”Eur.J.Pharmacol.. 407. 123-129 (2000)

Satoh H.: "Cardiac functions and taurine' s actions at different extra cellular calcium concentrations in forced swimming rats"Biol. Trac. Ele. Res. (in press). (2002)

Satoh H.：“强迫游泳大鼠的心脏功能和牛磺酸在不同细胞外钙浓度下的作用”Biol。