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Application of the Microbial Population Analysis Methods for the Investigation of Sediment Transfer and Materials Conversion Functions

微生物种群分析方法在沉积物迁移和物质转化功能研究中的应用

基本信息

批准号：
17360254
负责人：
SATOH Hiroyasu
金额：
$ 10.57万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17360254/
关键词：
sediment complex microbial system population structure microbial marker coastal zone Tokyo Bay Ariake Bay

项目摘要

The applicability of microbial population to investigate the sediment transfer phenomena was investigated. Samples were obtained mainly from Ariake Bay. In 2005 and 2006, samples were obtained from 23 points in Ariake Bay, and horizontal distribution of beta proteobacterial ammonia oxidizing bacteria was studied by the T-RFLP method. It was found that some of the OTUs (operational taxonomic units) distributed along the counter-clockwise flow of influent from Chikugo-River, and others were found more in the central part of the Ariake-Bay. It has been reported by other researchers that the influent from Chikugo-River has a counterclockwise flow, and the present study gave the same outcome. In 2007, an intensive sampling was done on 29th September at 5 locations with a multiple-time-point sampling. The whole bacterial community in the sediment samples and the bottom water samples were analyzed by the T-RFLP method. Prior to the study, a new DNA extraction method using sonication was developed, to enhance the throughput of the analysis with minimum amount of sample. By the developed method, PCR products were obtained from most of the samples only by sonication and dilution. In the same way, PCR products were obtained from suspended solids in the samples. PCR-T-RFLP method was applied to investigate the behavior of microorganisms in the bottom water within the short period of time on the sampling day. The dynamic behavior of the microbial population in the bottom water and its suspended solids samples were observed by the developed method. But unfortunately, in the present study, clear relationship between the sediment transfer and the microbial population change could not be found. It may be necessary to find key bacterial species that are suitable as a tracer. In conclusion, we developed a method to use microbial population structure to monitor the behavior of sediment and its transfer.

研究了微生物种群对沉积物迁移现象的适用性。样品主要采自有明湾。2005年和2006年，在Ariake Bay的23个采样点取样，采用T-RFLP法研究了β变形杆菌氨氧化菌的水平分布。结果表明，部分otu沿赤果河逆时针方向分布，部分otu主要分布在有良湾中部。据其他研究人员报道，chikugo河的流入具有逆时针流动，本研究也给出了相同的结果。2007年9月29日在5个地点进行了密集抽样，并进行了多时间点抽样。采用T-RFLP法对沉积物样品和底水样品中的细菌群落进行了整体分析。在此之前，研究人员开发了一种新的DNA提取方法，利用超声技术，以最少的样品量提高分析的通量。采用该方法，大多数样品只需超声和稀释即可获得PCR产物。用同样的方法，从样品中的悬浮固体中获得PCR产物。采用聚合酶链反应- t - rflp法，对采样当天短时间内底水中微生物的行为进行了研究。采用该方法对底水及其悬浮物样品中微生物种群的动态行为进行了观察。但遗憾的是，在目前的研究中，沉积物迁移与微生物种群变化之间没有明确的关系。可能有必要找到适合作为示踪剂的关键细菌种类。总之，我们开发了一种利用微生物种群结构监测沉积物及其转移行为的方法。