THE ROLE OF AMPHID OF S. RATTI LARVAE FOR MIGRATION IN RATS

在琼脂糖平板上制备0 - 400 mM的氯化钠梯度后，将棒状圆线虫感染性幼虫以0 mM和300 mM置于平板上。当置于300 mM浓度时，幼虫迅速避开并向低浓度区移动。另一方面，当将幼虫置于0 mM浓度下时，它们将浓度移动100 mM的范围，然后在平板上绘制环迹并返回。然而，当S.用透明质酸酶、刀豆蛋白A、胰蛋白酶、脂肪酶、NaIO_4等酶、凝集素和化学物质处理后，在300 mM琼脂糖平板上，它们表现出定向运动。这被认为是幼虫的感觉器官被上述化学物质破坏。这些感觉器官被认为存在于幼虫的端头动物中。然后将这种感觉器官破坏方法应用于幼虫的体内迁移。S.用35 S-蛋氨酸标记响尾蛇幼虫， ...更多信息 用不同浓度的透明质酸酶、刀豆蛋白A、胰蛋白酶、蛋白酶、脂肪酶和NaIO_4处理。感染后10、15、20天处死大鼠，用全身放射自显影法检测脑内移行幼虫数。但幼虫向鼻区和颅腔移动的数量与对照组相比无显著性差异。这些结果表明，幼虫的迁飞。体内感受器官与体外感受器官对NaCl的吸收作用不同。在前期研究的基础上，发现感染鼠先向鼻腔侵入，然后向头部侵入，标记幼虫感染鼠头皮下，分别于10、15、20 h后处死。幼虫头部似乎移动鼻尖，下腹部的对照幼虫（SC）稀疏地向前、后和内部移动。然后用同样的方法感染不确定的宿主豚鼠，分别于感染后15、25天处死动物。但头部感染时，所有幼虫都不会移到鼻腔。这些结果表明，S.响尾蛇幼虫具有严格的寄主特异性，对钠离子不产生反应，对体内其他器官物质也不产生反应。少

After making sodium chloride gradient between 0 〜 400mM on agarose plates, Strongyloides ratti infective larvae were placed on the plates at 0mM and 300mM. When placed at 300mM concentration, the larvae quickly avoided and moved to lower concentration area. On the other hand, When the larvae were placed at 0mM concentration, they moved the concentration by the extent of 100mM and then turned back with drawing loop traces on the plates. However, When S. ratti larvae had previously treated with various enzymes, lectins, and chemicalssuch such as hyaluronidase, Con A, trypsin, lipase, NaIO4 in vitro, they revealed an inderectional movements when placed on agarose plates at 300 mM consentration. This was considered for the larvae to be destroyed sense organs by the above chemicals. These sense organs are thought to exist in the amphids of the larvae. Then we applied this sensory organ destruction methods for larval migration in vivo. S. ratti larvae were labeled with 35S-methionine and the … More n they were treated by Hyaluronidase, Con A, Trypsin, Protease, Lipase and NaIO4 at various concentrations. Then larvae were infected to rats and the rats were killed at 10, 15, 20 post infection and number of larvae migrated in the cranial cavitied was examined by whole body autoradiography. However number of larvae were not significantly moved to nasal area and cranial cavity as compered to untreated control rats. These results imply that larval migration of S. ratti in vivo enployed by quite different sensory organs from those attracting NaCl in vitro. Next our previous data, infected rats seemed to orient to nasal cavity before invation to cranial, labeled larvae were infected subcutaneous site of rat heads and killed 10,15, 20h later. Al larvae in head portions seemed to move tip of the nose while. control larvae in lower abdominal site(sc) moved sparsely to anterior, posterior and internal portions. Next, same larvae were infected by same way above to indefinitive host, guinea pigs, and the animals were killed at 15, 25 after infection. However, all larvae won't move to nasal cavity when infected in head portion. These results meant that the sensory organ of amphids in S. ratti larvae had strict, host specificity and never responded only soduim ion but any other organ substances in vivo. Less