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THE ROLE OF AMPHID OF S. RATTI LARVAE FOR MIGRATION IN RATS

鼠鳉幼虫两栖动物对大鼠迁徙的作用

基本信息

批准号：
12670233
负责人：
KOGA Masataka
金额：
$ 2.11万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670233/
关键词：
Strongyloides ratti chemoattraction In vivo behavior interruption of larval migration lectin Invasion of nasal cavity gyunia-pigs autoradiography Chemoattraction 幼虫移行阻害 Chemotaxis mammal's sera albumine peptides ナトリウムイオンオートラ

项目摘要

After making sodium chloride gradient between 0 〜 400mM on agarose plates, Strongyloides ratti infective larvae were placed on the plates at 0mM and 300mM. When placed at 300mM concentration, the larvae quickly avoided and moved to lower concentration area. On the other hand, When the larvae were placed at 0mM concentration, they moved the concentration by the extent of 100mM and then turned back with drawing loop traces on the plates. However, When S. ratti larvae had previously treated with various enzymes, lectins, and chemicalssuch such as hyaluronidase, Con A, trypsin, lipase, NaIO4 in vitro, they revealed an inderectional movements when placed on agarose plates at 300 mM consentration. This was considered for the larvae to be destroyed sense organs by the above chemicals. These sense organs are thought to exist in the amphids of the larvae. Then we applied this sensory organ destruction methods for larval migration in vivo. S. ratti larvae were labeled with 35S-methionine and the … More n they were treated by Hyaluronidase, Con A, Trypsin, Protease, Lipase and NaIO4 at various concentrations. Then larvae were infected to rats and the rats were killed at 10, 15, 20 post infection and number of larvae migrated in the cranial cavitied was examined by whole body autoradiography. However number of larvae were not significantly moved to nasal area and cranial cavity as compered to untreated control rats. These results imply that larval migration of S. ratti in vivo enployed by quite different sensory organs from those attracting NaCl in vitro. Next our previous data, infected rats seemed to orient to nasal cavity before invation to cranial, labeled larvae were infected subcutaneous site of rat heads and killed 10,15, 20h later. Al larvae in head portions seemed to move tip of the nose while. control larvae in lower abdominal site(sc) moved sparsely to anterior, posterior and internal portions. Next, same larvae were infected by same way above to indefinitive host, guinea pigs, and the animals were killed at 15, 25 after infection. However, all larvae won't move to nasal cavity when infected in head portion. These results meant that the sensory organ of amphids in S. ratti larvae had strict, host specificity and never responded only soduim ion but any other organ substances in vivo. Less

琼脂糖板上使氯化钠梯度在0 ~ 400mM之间后，在0mM和300mM处放置rattyloides侵染幼虫。当浓度为300mM时，幼虫迅速躲避并迁移到浓度较低的区域。另一方面，当浓度为0mM时，幼虫将浓度移动100mM，然后在板上画出环形痕迹。然而，当在体外用各种酶、凝集素和化学物质（如透明质酸酶、Con A、胰蛋白酶、脂肪酶、NaIO4）处理过的黑氏葡萄球菌幼虫，在琼脂糖板上以300 mM浓度放置时，它们显示出间接运动。这被认为是上述化学物质破坏了幼虫的感觉器官。这些感觉器官被认为存在于幼虫的两栖动物中。然后，我们将这种感觉器官破坏方法应用于幼虫的体内迁移。分别用35s -蛋氨酸和35s -蛋氨酸进行标记，并用不同浓度的透明质酸酶、Con A、胰蛋白酶、蛋白酶、脂肪酶和NaIO4进行处理。分别于感染后10、15、20处死大鼠，采用全身放射自显影法观察幼虫在颅腔内迁移的数量。然而，与未处理的对照组相比，幼虫向鼻区和颅腔移动的数量不明显。这些结果表明，鼠形霉在体内的幼虫迁移所利用的感觉器官与在体外吸引NaCl的感觉器官有很大不同。根据我们之前的数据，感染的大鼠在侵入颅骨之前似乎是鼻腔的，标记的幼虫感染大鼠头部皮下部位，并在10、15、20小时后杀死。头部部分的幼虫似乎移动了鼻尖。下腹部（sc）的对照幼虫稀疏地向前、后、内移动。然后，用同样的方法将相同的幼虫感染给不确定的宿主豚鼠，在感染后15、25岁时杀死动物。然而，当头部感染时，所有的幼虫都不会移动到鼻腔。这些结果表明，蛇斑绦虫幼虫的感觉器官具有严格的寄主特异性，在体内并不只对钠离子有反应，而是对其他器官物质有反应。少