Ovarian Toxicity --Investigation of history of exposure to chemicals in infertility patients and an experimental study on a candidate chemical--

卵巢毒性--不孕症患者化学品接触史调查及候选化学品的实验研究--

• 批准号: 12670353
• 负责人: KAMIJIMA Michihiro
• 金额: $ 1.6万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670353/
• 关键词: Infertility; Ovary; Reproductive toxicity; Estrous cycle; Ovarian follicle; Organic solvent; Semen; Insecticide; 化学物質; 放射線

1.Investigation on occupational histories of female outpatients of infertility clinics.We randomly selected couples who visited infertility clinics, and asked them about their history of exposure to chemicals, physical agents and so on. Two hundred thirty females whose diagnosis was established were analyzed in the foiiowing two ways.1)After omitting patients who suffered from other causes of infertility than environmental factors, we analyzed the remaining 182 patients. Each environmental exposure was compared between hypothalamus/pituitary/ovarian axis(HPO)-impaired 80 patients and 102 other disease patients/normal spouses(free from gynecological disease).Significant difference in the radio of patients with solvent exposure history was observed between the groups(p<0.05 by chi-square test).2)The patients were divided into two groups, HPO-impaired 91 patients and 136 other disease patients/normal spouses (controls). Odds ratio of solvent exposure was 4.5(95% CI : 1.5-16.8).This study suggested the possible association of solvent exposure and to identfy more details of exposure and to explain the number of subject.2. Study of inhalation exposure to 1-bromopropane in female rats.Forty non-pregnant Wistar-strain rats were divided into 4 groups and each group was exposed to 0,200,400 or 800 ppm 1-bromopropane for 8 hours a day, 7 days a week, for 12 weeks. Vaginal smear tests showed dose-dependent disruption of eatrous cycle in 400 ppm or above concentrations. Primordial follicles was increased siginificantly in 800 ppm group, growing follicles decreased in 400 ppm or above concentrations, and antral follicles decreased in 200 ppm or above concentrations. This study shows that 1-bromopropane impaired the ovary and suggested that target follicles were different from those impaired by 2-brmopropane which induced apoptosis in primordial follicles.

1.不孕症门诊女性患者职业史的调查：随机抽取不孕症门诊就诊的夫妇，询问其接触化学、物理等物质的史，对确诊的230例女性进行以下两方面的分析：（1）排除环境因素以外的其他原因导致不孕的患者，（2）排除环境因素导致不孕的患者，（3）排除环境因素导致不孕的患者，（4）排除环境因素导致不孕的患者，（5）排除环境因素导致不孕的患者。我们分析了其余182名患者。每种环境暴露在80名下丘脑/垂体/卵巢轴（HPO）受损患者和102名其他疾病患者/正常配偶之间进行比较（无妇科疾病），两组间有溶剂接触史的比例有显著性差异（2）将患者分为两组，HPO受损组91例，其他疾病患者/正常配偶（对照组）136例。溶剂暴露的比值比为4.5（95%CI：1.5-16.8），本研究提示溶剂暴露可能存在相关性，并进一步明确暴露的细节和解释受试者人数.雌性大鼠吸入1-溴丙烷的研究40只未孕Wistar系大鼠被分为4组，每组分别吸入0、200、400和800 ppm的1-溴丙烷，每天8小时，每周7天，共12周。阴道涂片试验显示，在400 ppm或更高浓度下，食周期的破坏呈剂量依赖性。800 ppm组原始卵泡数显著增加，400 ppm及以上浓度组生长卵泡数减少，200 ppm及以上浓度组有腔卵泡数减少。本研究表明，1-溴丙烷损害卵巢，并建议，目标卵泡是不同的2-溴丙烷，诱导原始卵泡凋亡的损害。

项目成果

Gotoh Masahiro: "The changes of cholinesterase activity, nerve conduction velocity, and clinical signs and symptoms in termite control operators exposed to chlorpyrifos."Journal of Occupational Health. 43. 157-164 (2001)

Gotoh Masahiro：“接触毒死蜱的白蚁防治人员的胆碱酯酶活性、神经传导速度以及临床体征和症状的变化。”职业健康杂志。

Yu, Xiaozhong: "Involvement of Bcl-2 family genes and Fas signaling system in primary and secondary male germ cell apoptosis induced by 2-bromopropane in rat"Toxicology and Applied Pharmacology. 174. 35-48 (2001)

余晓忠：“Bcl-2家族基因和Fas信号系统参与2-溴丙烷诱导大鼠原发性和继发性雄性生殖细胞凋亡”毒理学和应用药理学。

Gotoh, Masahiro: "The changes of cholinesterase activity, nerve conduction velocity, and clinical signs and symptoms in termite control operators exposed to chlorpyrifos"Journal of Occupational Health. 43. 157-64 (2001)

Gotoh，Masahiro：“接触毒死蜱的白蚁防治操作人员的胆碱酯酶活性、神经传导速度以及临床体征和症状的变化”职业健康杂志。

竹内 康浩: "化学物質による健康障害とリスクアセスメント"労働と健康. 27. 1-6 (2001)

Yasuhiro Takeuchi：“化学物质造成的健康危害和风险评估”《劳工与健康》27. 1-6 (2001)。

Wang, Hailan: "Biochemical changes in the central nervous system of rats exposed to 1-bromopropane for seven days"Toxicological Sciences. (in press). (2002)

王海兰：“大鼠接触1-溴丙烷7天后中枢神经系统的生化变化”毒理学。