Role of gastric leptin and cholecystokinin in patients with functional dyspepsia

胃瘦素和胆囊收缩素在功能性消化不良患者中的作用

• 批准号: 12670536
• 负责人: YOSHIKAWA Ichiro
• 金额: $ 1.79万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670536/
• 关键词: Functional dyspepsia; Leptin; Cholecystokinin

The patients with functional dyspepsia and healthy volunteers were investigated in the study. They all gave written informed consent for blood sampling and gastric mucosal biopsy by endoscopy. This study was approved by the Ethics Committee of our institute. Plasma CCK and leptin were estimated using a RIA kit. Biopsies of fundic sites were performed during endoscopic examination. Immunostaining for leptin was performed by a polyclonal rabbit antihuman leptin antibody (A-20, Santa Cruz Biotechnology, California, USA). Neither plasma CCK nor leptin levels differed significantly between the patients with functional dyspepsia and healthy volunteers. Leptin immunoreactive cells were found in the lower half of the epithelial glands of the corps. Intensity of positive staining for leptin in the epithelial cells was rated on a 5-point score. There was no significant difference in intensity of positive staining for leptin between the patients with functional dyspepsia and healthy volunteers. In addition, there was no significant difference in intensity of positive staining for leptin between the H. pylori-negative and -positive groups, between the high (≧23) and low (<23) levels of body mass index groups or between the aged (≧70) and not aged (<70) groups. Further determination of gastric mucosal leptin mRNA and duodenal mucosal CCK mRNA was required.

该研究对功能性消化不良患者和健康志愿者进行了调查。他们都签署了通过内窥镜进行血液采样和胃粘膜活检的书面知情同意书。本研究经我所伦理委员会批准。使用 RIA 试剂盒估算血浆 CCK 和瘦素。在内窥镜检查期间进行胃底部位的活组织检查。通过多克隆兔抗人瘦素抗体（A-20，Santa Cruz Biotechnology，加利福尼亚州，美国）对瘦素进行免疫染色。功能性消化不良患者和健康志愿者之间的血浆 CCK 和瘦素水平均无显着差异。在军团上皮腺的下半部发现了瘦素免疫反应细胞。上皮细胞中瘦素阳性染色的强度按 5 分评分进行评级。功能性消化不良患者与健康志愿者之间瘦素阳性染色强度无显着差异。此外，幽门螺杆菌阴性组和阳性组之间、高体重指数组(≥23)和低体重指数组(<23)之间、老年组(≥70)和非老年组(<70)之间瘦素阳性染色强度没有显着差异。需要进一步测定胃粘膜瘦素mRNA和十二指肠粘膜CCK mRNA。

项目成果

I.Yoshikawa, M.Otsuki, et al.: "Serum pepsinogen can predict response to H2-receptor antagonist in patients with functional dyspepsia"Alimentary Pharmacology and Therapeutics. 16巻・10号. 1805-1809 (2002)

I. Yoshikawa、M. Otsuki 等人：“血清胃蛋白酶原可以预测功能性消化不良患者对 H2 受体拮抗剂的反应”《消化药理学和治疗学》第 16 卷，第 10 期。1805-1809 (2002)。

Yoshikawa, et al.: "Serum pepsinogen can predict response to H2-receptor antagonist in patients with functional dyspepsia"Alimentary Pharmacology and Therapeutics. 16巻・10号. 1805-1809 (2002)

Yoshikawa 等人：“血清胃蛋白酶原可以预测功能性消化不良患者对 H2 受体拮抗剂的反应”《消化药理学和治疗学》第 16 卷，第 10 期。1805-1809 (2002)

Ichiro Yoshikawa: "Serum pepsinogen can predict response to H2-receptor antagonist in patients with functional dyspepsia"Alimentary Pharmacology and Therapeutics. 16-10. 1805-1809 (2002)

Ichiro Yoshikawa：“血清胃蛋白酶原可以预测功能性消化不良患者对 H2 受体拮抗剂的反应”《消化药理学和治疗学》。