RESEARCH OF THE STRUCTURE AND BINDING BETWEEN T CELL RECEPTOR AND ANTIGEN-PRESENTING MOLECULE IN THE ALLERGIC DISEASE

(1)从5例患者建立的6个TCC对3个不同的肽片段有反应，其中BLGp97-117片段被T细胞识别的频率最高。BLGp101-112(KYLLFCMENSAE)是该区域识别的核心序列。(2)牛奶过敏组的HLADRB1^*0405等位基因携带率高于对照组，但两组间差异无统计学意义。(3)RT-PCRTCR序列分析显示CDR3区氨基酸序列具有明显的异质性，且存在相似的氨基酸序列模式。(4)细胞因子分析显示ALL抗原特异性T细胞株产生高水平的IL-5，这些T细胞株也不同程度地产生IL-4和干扰素-γ。IL-4与干扰素-γ比值的差异可能是其临床表现不同的原因。(5)我们用另一种氨基酸取代了BLGp101-111的一个氨基酸，合成了几个类似的多肽。T细胞克隆只与具有相似性质的多肽发生反应。抗原的生化特性可以规定T细胞在变态反应发病机制中的反应。我们的目标是开发使用类似肽来诱导Th1型T细胞反应的新疗法。

(1) Six TCCs established from the five patients responded to three different peptide fragments, and the fragment of BLGp97-117 was recognized most frequently by the T cells. BLGp101-112 (KYLLFCMENSAE) was the core sequence to be recognized in this portion.(2) Milk allergy group was higher than control group in possession ratio of HLA-DRB1^*0405 allele, but there was not significant difference between both groups. HLA-DRB1^*0405 allele participate in BLG-recognition profoundly.(3) Sequence analysis of TCR with RT-PCR method revealed a striking heterogeneity and similar patterns of amino acid sequence were recognized in the CDR3 region.(4) Analysis of cytokine production revealed high levels of IL-5 production by antigen-specific T cell lines of all ; IL-4 and IFN-γ were also produced in a variety of degree by these T cell lines. The differences in the ratios of IL-4 to IFN-γ might explain the differences in their clinical features.(5) We substituted another amino acid for an amino acid of BLGp101-111 by one and composed several analogue peptides. The T cell clone reacted to only the peptides with similar property. Biochemical property of the antigen can prescribe the T cell response in the allergic patho-genesis. We aim development of the novel therapy using analogue peptides that induce Th1-typed T-cell response.