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Characteristics of apoptosis in the brainstem of neonatal SIDS victims

新生儿SIDS患者脑干细胞凋亡特征

基本信息

批准号：
12670783
负责人：
SAWAGUCHI Toshiko
金额：
$ 2.11万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670783/
关键词：
neonate SIDS brainstem apoptosis 新生児SIDS TUNEL

项目摘要

To investigate the difference and/or similarity of pathophysiology between post-neonatal SIDS victims and neonatal SIDS victims, the characteristics of apoptosis in the brainstem was studied in this report.The brainstems of twenty-six postneonatal SIDS cases and of twelve control cases were collected in Brussels Free University Children's Hospital Reine Fabiola. The brainstems of six neonatal SIDS cases and three control cases including two cases of which causes of deaths were pulmonary hemorrhage and one case of 2-month old SIDS were collected in Japan. For postneonatal SIDS cases, terminal deoxynucleotidyl nick end labelling (TUNEL) method was carried out to investigate apoptosis and immunohistochemistry of Gap43 was carried out to investigate neuronal plasticity For neonatal SIDS cases, TUNEL method and immunohistochemistry of active Caspase-3 and bcl-2 were carried out to investigate apoptosis. The results were manually counted and the positive cell density was semi-quantitized.In the post-neonatal SIDS cases, the density of TUNEL-positive glias in pedunculopontine tegmentum nuclei was associated with the frequency of obstructive sleep apnea and also correlated with the average number of spines of Gap43-positive neurons in pedunculopontine tegmentum nuclei.As for the neonatal SIDS cases, the cell density of TUNEL positive neurons and Gap43-positive neurons in control cases with pulmonary haemorrhage was slightly higher than in those in neonatal SIDS victims. Also in the neonatal SIDS cases and the 2 month old SIDS case, the cell density of TUNEL positive glias and Gap43-positive glias particularly in the pontine nuclei was higher than in control cases with pulmonary haemorrhage. These similarities were observed in neonatal SIDS cases and the 2 month old SIDS case but no clear different tendency between those were not observed.

为了探讨新生儿后SID患者与新生儿SID患者在病理生理学上的差异和/或相似性，本文研究了新生儿后SID患者脑干细胞凋亡的特征。在日本收集了6例新生儿小儿麻痹症病例和3例对照病例的脑组织，其中2例死因为肺出血，1例为2月龄小儿麻痹症。采用末端脱氧核苷酸缺口末端标记法(TUNEL)检测细胞凋亡，免疫组织化学方法检测GAP43蛋白表达，观察新生儿SIDs神经元的可塑性。采用原位末端标记法和免疫组织化学方法检测细胞凋亡。结果人工计数，阳性细胞密度半定量。在新生儿SIDS病例中，桥脑脚盖核TUNEL阳性胶质细胞密度与阻塞性睡眠呼吸暂停的发生有关，也与桥脑盖核GAP43阳性神经元的平均棘数相关。新生儿SIDs和2月龄SIDSTUNEL阳性胶质细胞和GAP43阳性胶质细胞密度均高于对照组，尤以桥核为甚。新生儿和2个月大的SID病例有相似之处，但无明显不同趋势。