Analysis of modulation of expression of cellular prion-related protein by skin immune sells

皮肤免疫信号对细胞朊病毒相关蛋白表达的调节分析

• 批准号: 12670809
• 负责人: NAKAMURA Koichiro
• 金额: $ 1.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670809/
• 关键词: skin; Langerhans cells; prion; Thy-1 dendritic epidermal T cells; CD40; TNF-α; プリオン

The modulation of expressions of cellular prion-related protein was examined in skine immune cells such as ep demal Langerhans cells (LC) and kesahisocyles (KC). The presence of prp^c on LC,DC in the skin, and KC was demonstrated in immunohis to chenical stauning, immunoblot and FACS, PrPc knock out mice showed negative PrPC expression on these cells and γδ^+TCR dendritic epidemal T cells (DETC ).In uitro examination revealed that TNFα, IL-1β uprequlated R-P^c expresnin on LC, and GM-CSF down requlated this expression. These data suggist that LC and KC may be the targets for peripheral in fection with prion proteins.

在皮肤免疫细胞如表皮朗格汉斯细胞（LC）和毛细胞（KC）中检测细胞朊病毒相关蛋白表达的调节。免疫组化染色、免疫印迹和流式细胞仪检测结果表明，在LC、皮肤DC和KC上存在PrPC，PrPC基因敲除小鼠的这些细胞和γδ^+TCR树突状T细胞（DETC）上均不表达PrPC，体外检测结果表明，TNFα、IL-1β可上调LC上R-P^c的表达，而GM-CSF可下调R-P^c的表达。提示LC和KC可能是朊病毒感染的靶细胞。

项目成果

Tada Y, et al: "Granulocyte/macrophage colony stimulating factor inhibits IL-12 production of mouse Langerhans cells."J Immunol. 164. 5113-5119 (2000)

Tada Y 等人：“粒细胞/巨噬细胞集落刺激因子抑制小鼠朗格汉斯细胞的 IL-12 产生。”J 免疫学杂志。

Tada Y, et al.: "Transforming growth factor-β upregulates CD40-engaged IL-12 Production of mouse Langerhans cells"Eur J Immunol.. 31. 294-300 (2001)

Tada Y 等人：“转化生长因子-β 上调 CD40 接合的小鼠朗格汉斯细胞的 IL-12 产生”Eur J Nutrition.. 31. 294-300 (2001)

Wakugawa, Nakamura K, et al.: "CC chemokine receptor 4 expression on peripheral blood CD4+T cells reflects disease activity of atopic dermatitis"Journal of Investigative Dermatology. 117. 188-196 (2001)

Wakukawa、Nakamura K 等人：“外周血 CD4 T 细胞上的 CC 趋化因子受体 4 表达反映了特应性皮炎的疾病活动性”《皮肤病学研究杂志》。

Watanabe T, Nakamura K, et al.: "Antibodies to mollus cam contagiosum virus in the general population and susceptible patients"Archives of Dermatology. 136. 1518-1522 (2001)

Watanabe T、Nakamura K 等人：“普通人群和易感患者中传染性软体动物病毒的抗体”皮肤病学档案。

Wakugawa, Nakamura K, et al.: "CC Chemokine receptor 4 expression on peripheral blood CD40 T-cells reflects disease activity of atropic dermatitis"Journal of Investigative Dermatology. 117. 188-196 (2001)

Wakukawa、Nakamura K 等人：“外周血 CD40 T 细胞上的 CC 趋化因子受体 4 表达反映了特应性皮炎的疾病活动性”《皮肤病学研究杂志》。