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Cytokine, apoptosis, and homing receptor in acute exanthematous viral infe

急性发疹病毒感染中的细胞因子、细胞凋亡和归巢受体

基本信息

批准号：
12670834
负责人：
TERAKI Yuichi
金额：
$ 1.98万
依托单位：
Kyorin University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670834/
关键词：
Measles T cell Cytokine CLA ホーミング急性ウイルス発疹症

项目摘要

recent evidence suggests that the γδT cells are key players in the regulation of innate and acquired immune responses; however, a little information is available how γδT cells coordinate the interplay of innate and acquired immune responses in viral infections in human. We therefore, investigated the kinetics of immune responses of γδT cells and CD8+ T cells as well as those of NK cells and CD4+ T cells during measles virus infection, by flow cytometry. In the peripheral blood of patients with measles significant numbers of CD69 (activation marker) expressing -γδ T cells and -NK cells were observed as early as 1〜3 days after the onset of disease. CD69 expressing-CD8+ T cells were significantly increased in number accompanied by decreases in CD69 expressing-γδT cells and -NK cell numbers at 3〜5 days after the onset. CD69 expressing -γδT cells, -NK cell, and -CD8+ T cells were almost diminished at 5〜8 days after the onset. There was no significant increase in CD69 expressing-CD4+ T cell number during measles infection. A majority of γδT cells and NK cell were populations capable of producing IFN-γ. IFN-γ producing-CD8+ T cells were significantly increased in number at 3〜5 days after diagnosis, and a majority of these cells expressed CLA. On the other hand , IL-10 producing-CD4+ T cells were significantly increased in number at 3〜5 days after diagnosis. These findings suggest that there are biphasic protective immune responses by γδT cells and CD8+ T cells in measles virus infection. Additionally, CD4+ T cells may work as a regulatory cell in the inflammatory responses of measles virus infection.

最近的证据表明，γδT细胞是调节先天性和获得性免疫应答的关键参与者;然而，在人类病毒感染中，γδT细胞如何协调先天性和获得性免疫应答的相互作用的信息很少。因此，我们用流式细胞术研究了麻疹病毒感染过程中γδT细胞和CD 8 + T细胞以及NK细胞和CD 4 + T细胞免疫应答的动力学。在麻疹患者的外周血中，早在发病后1 ~ 3天就观察到大量表达CD 69（活化标志物）的-γδ T细胞和-NK细胞。在发病后3 ~ 5天，表达CD 69的-CD 8 + T细胞数量显著增加，同时表达CD 69的-γδT细胞和-NK细胞数量减少。发病后5 ~ 8d，表达CD 69的-γδT细胞、-NK细胞和-CD 8 + T细胞几乎减少。在麻疹感染过程中，表达CD 69的CD 4 + T细胞数量没有显著增加。大部分γδT细胞和NK细胞是能够产生IFN-γ的群体。在诊断后3 ~ 5天，产生IFN-γ的CD 8 + T细胞的数量显著增加，并且这些细胞中的大多数表达CLA。另一方面，IL-10产生-CD 4 + T细胞的数量在诊断后3 - 5天显著增加。提示麻疹病毒感染时存在γδT细胞和CD 8 + T细胞的双相保护性免疫应答。此外，CD 4 + T细胞可能在麻疹病毒感染的炎症反应中起调节细胞的作用。