Chronological gene expression profile in acute renal failure using CDNA array method

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1.Non-RI CDNA array method was established utilizing low cycle RT-PCR.2.In vitro hypoxia reoxyganetion model was established using human proximal epithelial cells. Time points that distinguish reversible from irreversible damage was determined.3.Mice renal ischemia-reperfusion model was established as in vivo model.4.Gene profiles in reversible/irreversible damage from hypoxia reoxyganetion were obtained using non-RI CDNA array.5.Subgroup of genes that increase expression during reversible phase and decrease in irreversible damage are focused-as the marker genes prophetic of reversibility in in vitro model.6.Subgroup of genes that increase expression during reversible phase and decrease in irreversible damage are focused as the marker genes prophetic of reversibility in in vivo model.