Chronological gene expression profile in acute renal failure using CDNA array method

使用 cDNA 阵列方法分析急性肾衰竭的时间基因表达谱

• 批准号: 12671026
• 负责人: NOIRI Eisei
• 金额: $ 2.18万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671026/
• 关键词: Ischemia-reperfusion; Acute renal failure; Human proximal tubular epithelial cells; Hypoxic stress; Macro-array method; non RI; 虚血再灌流腎障害; 虚血再灌流; 低酸素-再酸素化; cDNAアレイ法

1.Non-RI CDNA array method was established utilizing low cycle RT-PCR.2.In vitro hypoxia reoxyganetion model was established using human proximal epithelial cells. Time points that distinguish reversible from irreversible damage was determined.3.Mice renal ischemia-reperfusion model was established as in vivo model.4.Gene profiles in reversible/irreversible damage from hypoxia reoxyganetion were obtained using non-RI CDNA array.5.Subgroup of genes that increase expression during reversible phase and decrease in irreversible damage are focused-as the marker genes prophetic of reversibility in in vitro model.6.Subgroup of genes that increase expression during reversible phase and decrease in irreversible damage are focused as the marker genes prophetic of reversibility in in vivo model.

1.利用低周期RT-PCR2建立非RI型CDNA阵列方法。2.利用人近端上皮细胞建立体外缺氧复氧模型。3.建立小鼠肾缺血再灌注模型。4.利用非RI型CDNA阵列获得缺氧再给氧引起的可逆/不可逆损伤的基因图谱。5.在体外模型中聚焦可逆期表达增加而不可逆性损伤减少的基因亚群--作为可逆性的标记基因。6.在体内模型中聚焦可逆期表达增加而不可逆性损伤减少的标记基因亚群。

项目成果

Yamamoto T, Tada T, Brodsky SV, Tanaka H, Noiri E, Kajiya F, Goligorsky MS: "Intrayital Videomicroscopy of peritubular capillaries in renal ischemia"Am J Physiol Renal Physiol. epub ahead of print. (2002)

Yamamoto T、Tada T、Brodsky SV、Tanaka H、Noiri E、Kajiya F、Goligorsky MS：“肾缺血中管周毛细血管的腔内视频显微镜检查”Am J Physiol Renal Physiol。

Noiri E, Nakao A, Uchida K, Tsukahara H, Ohno M, Fujita T, Brodsky S, Goligorsky M: "Scavenging of peroxynitrite ameliorates lipid peroxidation and DNA damage in experimental ischemic acute renal failure"Am J Physiol Renal Physiol. 281. F948-F957 (2001)

Noiri E、Nakao A、Uchida K、Tsukahara H、Ohno M、Fujita T、Brodsky S、Goligorsky M：“清除过氧亚硝酸盐可改善实验性缺血性急性肾衰竭中的脂质过氧化和 DNA 损伤”Am J Physiol Renal Physiol。

Usui T, Hara M, Satoh H, Moriyama N, Kagaya H, Amano S, Oshika T, Ishii Y, Ibaraki N, Hara C, Kunimi M, Noiri E, Tsukamoto K, Inatomi J, Kawakami H, Endou H, Igarashi T, Goto A, Fujita T, Araie M, Seki G.: "Molecular basis of ocular abnormalities associat

臼井 T、原 M、佐藤 H、森山 N、加贺屋 H、天野 S、大石 T、石井 Y、茨木 N、原 C、国见 M、野里 E、冢本 K、稻美 J、川上 H、远藤 H、五十岚 T

Goligorsky MS, Brodsky SV, Noiri E: "Nitric oxide in acute renal failure : NOS versus NOS"Kidney Int. (in press). (2002)

Goligorsky MS、Brodsky SV、Noiri E：“急性肾衰竭中的一氧化氮：NOS 与 NOS”Kidney Int。

Noiri E, Ozawa H, Fujita T, Nakao A: "Pharmacokinetics of cetirizine in chronic hemodialysis patients : multiple-dose study"Nephron. 89. 101-4 (2001)

Noiri E、Ozawa H、Fujita T、Nakao A：“慢性血液透析患者中​​西替利嗪的药代动力学：多剂量研究”Nephron。