Analysis of colon-specific unknown gene possibly associated with inflammatory bowel disease

可能与炎症性肠病相关的结肠特异性未知基因分析

• 批准号: 12671194
• 负责人: SHIBATA Chikashi
• 金额: $ 2.43万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671194/
• 关键词: intestinal flora; epithelial cells; syncollin; host defense; exocytosis

Background arid Aims : Intestinal microorganisms play role in inflammatory bowel disease. The aim of this study was to identify epithelial gene expression down-regulated by bacterial flora, and to examine expression of human homologue and possible role in minflammatory bowel disease.Methods : Germ-free mice were orally given bacterial suspension prepared from conventional components (bacterial reconstitution), and differential gene expression in intestinal epithelial cells was identified by differential display. Expression of the identified gene was examined in dextran sulfate-sodium induced colitis or human inflammatory bowel disease, by Northern blot and quantitative RT-PCR.Results : Syncollin mRNA was identified as a strongly down-regulated gene after bacterial reconstitution. In contrast, syncollin mRNA expression was stable in colonic epithelial cells of dextran sulfate-sodium -treated mice. Human syncollin mRNA deceased in UC but not CD epithelial cells in an independent manner of macroscopic inflammation.Conclusion : Since rat syncollin is an essential molecule in exocytotic pathway, decreased syncollin expression in UC may be associated with abnormal secretion.

背景和目的：肠道微生物在炎症性肠病中发挥作用。本研究的目的是鉴定细菌菌群下调的上皮基因表达，并研究人类同源基因的表达及其在炎症性肠病中的可能作用。方法：用常规成分制备的细菌悬浮液（细菌重组）口服无菌小鼠，用差异展示法鉴定肠上皮细胞的差异基因表达。通过Northern blot和定量RT-PCR检测该基因在葡聚糖硫酸钠诱导的结肠炎或人炎症性肠病中的表达。结果：细菌重组后，Syncollin mRNA被鉴定为一个强烈下调的基因。与此相反，葡聚糖磺酸钠处理小鼠结肠上皮细胞中syncollin mRNA表达稳定。人syncollin mRNA在UC上皮细胞中以独立的方式死亡，而在CD上皮细胞中不存在。结论：大鼠共联蛋白是胞外通路的重要分子，UC中共联蛋白表达减少可能与分泌异常有关。

项目成果

福島浩平 他: "Epithelial induction of Serum amyloid A in experimental mucogal in flammation"Digesfiul Diseases and Scisuces. (in press). (2002)

Kohei Fukushima 等人：“炎症中实验粘液中血清淀粉样蛋白 A 的上皮诱导”Digesfiul 疾病和剪刀（2002 年出版）。

Kouhei Fukushima, Hitoshi Ogawa, Taku Kitayama, Toshiyuki Yamada, Hiroo Naito, Yuji Funayama, Seiki Matsuno, Iwao Sasaki: "Epithelial induction of Serum amyloid A in experimental mucosal inflammation"Digestive Diseases and Sciences. (in press). (2002)

Kouhei Fukushima、Hitoshi Okawa、Taku Kitayama、Toshiyuki Yamada、Hiroo Naito、Yuji Funayama、Seiki Matsuno、Iwao Sasaki：“实验性粘膜炎症中血清淀粉样蛋白 A 的上皮诱导”消化疾病与科学。