The association of Epstein-Barr virus with gastric carcinoma and clinical application

EB病毒与胃癌的关系及临床应用

• 批准号: 12671201
• 负责人: ODA Kenji
• 金额: $ 1.28万
• 依托单位: CHIBA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671201/
• 关键词: Gastric carcinoma; Epsten-Barr virus; Anti-EBV anbtibody; Clinical application

The purpose of this study is to investigate the association of Epstein-Barr virus (EBV) with gastric carcinoma by polymerase chain reaction (PCR) and in situ hybridization (ISH) techniques, and also by the measurement of anti-EBV antibody titers. 1. Association of EBV with lymphoepithlioma-type gastric carcinoma : 12 of 13 cases were EBV-positive (92.3%). Southern blot analysis indicated the episomal form and monoclonality of EBV. 2. Association of EBV with ordinary type gastric carcinomas : One hundred and two cases of gastric carcinomas were studied. In informative 97 cases, EBV was detected in 21 cases (21.6%) by PCR method. ISH studies showed that the EBV RNA was expressed in 5 of 97 cases (5.2%) and localized to the nuclei of carcinoma cells. 3.Association of EBV with remnant gastric carcinomas : Three of 10 cases of remnant gastric carcinomas (30.0%) were EBV-positive. The decrease of acid secretion of remnant stomach may relate to EBV infection. 4.Clarification of the elevation of anti-EBV tilers in the EBV-related gastric cancer patients : Anti-EBV capsid antigen (VCA)-IgG, early antigen (EA)-IgG and EBV nuclear antigen (EBNA) liters were elevated in the EBV-related gastric cancer patients. Elevation of the anti-EA-IgG liter was most specific and we may use it as tumor marker for the EBV-relaled gastric cancer.

采用聚合酶链式反应（PCR）和原位杂交（ISH）技术，以及检测EBV抗体滴度，探讨eb病毒（EBV）与胃癌的关系。1. EBV与淋巴上皮瘤型胃癌的相关性：13例中EBV阳性12例（92.3%）。南方印迹分析表明，EBV具有个体形式和单克隆性。2. EBV与普通型胃癌的关系：对102例胃癌进行了研究。在97例病例中，PCR检出EBV 21例（21.6%）。ISH研究显示，97例患者中有5例（5.2%）表达EBV RNA，并定位于癌细胞细胞核。3.EBV与残胃癌的相关性：10例残胃癌中3例（30.0%）EBV阳性。残胃胃酸分泌减少可能与EBV感染有关。4.澄清EBV相关胃癌患者抗EBV抗体水平升高：EBV相关胃癌患者抗EBV衣壳抗原(VCA)-IgG、早期抗原(EA)-IgG、EBV核抗原（EBNA）升升高。抗ea - igg升高是最特异的，我们可以将其作为ebv相关性胃癌的肿瘤标志物。