Clinical utilization of profiles of cancer-related factors in gastric and esophageal cancer

胃癌和食管癌相关因素谱的临床应用

• 批准号: 12671231
• 负责人: NOSHIRO Hirokazu
• 金额: $ 2.11万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671231/
• 关键词: gastric cancer; cancer-related factors; pathological examination; tumor marker; esophageal cancer; 腫瘍増殖関連遺子; 遺伝子; 早期癌

<Object> To investigate whether profiles of gene expression of cancer-related factors in biopsy specimens of gastric cancer are possible to give suggestion to select diminished surgery as laparoscopic gastrectomy, and expression of such cancer-related factors is related to other prognostic factors. Furthermore, gene expression of such cancer-related gene in esophageal cancer and its relation to pathological feature of resected specimens were investigated.<Methods> In our series of 47 gastric cancer, mRNA expression of PDGFA, TGFbeta and MMP2 were examined. Additionally, mRNA expression of uPA, uPAR, VEGF, PDGFB, IL10, CyclinDl, CyclinE, COX2, erbB2 and INOS were evaluated in the former 13 cases. Afterward, relation between profiles of gene expression and clinicopathological features as depth of primary tumor, lymph node metastasis, CEA, CA19-9 was studied. In 5 esophageal cancer series, relation between gene expression of PDGFA, TGFbeta, MMP2, uPA, PAI-1 and VEGF in biopsy specimens an … More d such clinicopathological features were investigated.<Results> In our series of gastric cancer, gene expression of such cancer-related factors was not related significantly to depth of primary tumor and lymph node metastasis. However, gene expression of MMP2 is possible to suggest precise depth of primary tumor which preoperative examinations couldn't detect in some cases. Although there was not significant relations between gene expression and tumor markers as CEA and CA19-9, in all the cases without any gene expression, no elevation of these tumor markers was found. In the series of esophageal cancer, it was revealed that the depth of primary tumor was beyond the serosa (T3) in the cases in which 3 out of 6 factors were positive and the depth was superficial (T1b) in the cases in which only 1 or 2 factors were positive. In all 5 cases of esophageal cancer, there was no lymph node metastasis and no gene expression of VEGF. There was no significant relation between gene expression and tumor marker elevation.<Discussion and Perspective> Profile of gene expression of cancer-related factors are useful and beneficial to decide therapeutic strategies and provide information about prognosis because gene expression of several factors could make up for preoperative evaluation by conventional examinations in our series of gastric cancer, although relation between gene expression and clinicopathological features was not sufficiently significant. Current data regarding esophageal cancer shows that there will be significant relation between gene expression and clinicopathological features in esophageal cancer due to its high malignant potential. Therefore, it is worthwhile to continue this project. Less

<Object>探讨胃癌活检标本中癌相关因子的基因表达谱是否可作为腹腔镜胃切除术减容术的选择依据，以及这些癌相关因子的表达与其他预后因素的关系。并进一步研究这些癌相关基因在食管癌组织中的表达及其与食管癌病理特征的关系。<Methods>在我们的47例胃癌中，检测了PDGFA、TGF β和MMP 2的mRNA表达。检测uPA、uPAR、VEGF、PDGFB、IL 10、CyclinD 1、CyclinE、COX 2、erbB 2、INOS等基因mRNA的表达。然后研究基因表达谱与原发肿瘤深度、淋巴结转移、CEA、CA 19 -9等临床病理特征的关系。在5个食管癌系列中，PDGFA、TGF β、MMP 2、uPA、派-1和VEGF在活检标本中的基因表达与食管癌的发生、发展和预后的关系， ...更多信息 d这些临床病理特征进行了研究。<Results>在我们的胃癌系列中，这些癌症相关因子的基因表达与原发肿瘤的深度和淋巴结转移无关。然而，MMP 2基因表达可能提示术前检查在某些情况下无法检测到的原发肿瘤的精确深度。基因表达与肿瘤标志物CEA、CA 19 -9的表达无明显相关性，但在所有无基因表达的病例中，均未发现这些肿瘤标志物的升高。6项因子中有3项阳性的病例，原发肿瘤的深度超过浆膜（T3），深度为浅表（T1 b）。在只有1或2个因素为阳性的情况下。5例食管癌均无淋巴结转移，VEGF基因均无表达。基因表达与肿瘤标志物升高之间无显著相关性。<Discussion and Perspective>尽管基因表达与临床病理特征之间的关系并不十分显著，但在我们的系列胃癌中，几种癌相关因子的基因表达可以弥补常规检查对术前评估的不足，因此，对胃癌相关因子的基因表达谱的研究有助于制定治疗策略和提供预后信息。目前有关食管癌的资料显示，由于食管癌的高恶性潜能，基因表达与临床病理特征之间将有显着的关系。因此，继续这个项目是值得的。少

项目成果

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Sasaki N, Morisaki T, Hashizume K, Yao T, Tsuneyoshi M, Noshiro H, Nakamura K, Uchiyama A, Tanaka M, Katano M: "Nuclear factor kappa B/p65(RelA) is constitutive activation in human gastric carcinoma"Clinical Cancer Research. 7. 4136-4142 (2001)

Sasaki N、Morisaki T、Hashizume K、Yao T、Tsuneyoshi M、Noshiro H、Nakamura K、Uchiyama A、Tanaka M、Katano M：“核因子 kappa B/p65 (RelA) 是人类胃癌的组成型激活”临床癌症

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