MECHANISM OF CAR CINOGENESIS IN THE BILIATY SYSTEM-MOLECULAR BIOLOGICAL ANALYSIS OF PANCREATICOBILIARY MALJUNCTION USING CDNA MICROARRAY

胆道系统中汽车的发生机制——CDNA微阵列对胰胆管连接不良的分子生物学分析

• 批准号: 12671246
• 负责人: SEKIDO Hitoshi
• 金额: $ 2.11万
• 依托单位: YOKOHAMA CITY UNIVERSITY, SCHOOL OF MECICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671246/
• 关键词: Pancreaticobiliary maljunction; Micro array; cDNA; Carcinogenesis

The mechanism of gastrointestinal carcinogenesis was comprehensively analyzed using cDNA micro array. Materials were the cancerous or non-cancerous mucosa at the gallbladders or common bile ducts taken from surgical specimen of the patients with pancreaticobiliary maljunction (PBMJ). RNAs were extracted from these samples. Because the quantity of the RNA was considerably small, amplification was performed according to the linear amplification method using Amino MEGA Script T7 kit. Applying this technique, the enough RNAs were obtained form 1.5 to 3 micrograms of total RNA. The linearity analysis between before and after the amplification was performed and well correlation was confirmed, correlation coefficient 0.7. Comprehensive analyses of gene expressions were performed using RNA samples extracted from the surgical specimens in the patients with PBMJ. The changes of gene expression patterns were comprehensively detected by cDNA micro array. Comparing these changes between colonic adenomas and the biliary mucosae in PBMJ, this study may contribute to elucidating the mechanism of gastrointestinal carcinogenesis.

利用cDNA微阵列技术对胃肠道肿瘤的发生机制进行了综合分析。材料为胰胆管合流异常（PBMJ）患者手术切除的胆囊或胆总管癌性或非癌性粘膜。从这些样品中提取RNA。因为RNA的量相当少，所以根据使用Amino MEGA Script T7试剂盒的线性扩增方法进行扩增。应用该技术，从1.5至3微克总RNA中获得足够的RNA。对扩增前后的结果进行线性分析，相关系数为0.7。从PBMJ患者的手术标本中提取RNA样本进行基因表达的综合分析。利用cDNA微阵列技术全面检测基因表达谱的变化。比较PBMJ中结肠腺瘤和胆管粘膜的这些变化，有助于阐明胃肠道癌变的机制。

项目成果

Sekido H, Morita T, Matsuo K, Miura Y, Kunihiro O, Ichikawa Y, Endo I, Togo S, Shimada H,: "Follow-up After Biliary Reconstruction in Pancreaticobiliary Maljunction (PBMJ): Are There Any Differences Between in PBMJ And in Non-PBMJ?"5th World Congress of t

Sekido H、Morita T、Matsuo K、Miura Y、Kunihiro O、Ichikawa Y、Endo I、Togo S、Shimada H，：“胰胆管连接不良 (PBMJ) 胆道重建后的随访：PBMJ 和

Ichikawa Y, Kamiyama M, Sekido H, Ishikawa T, Miura Y, Kamiya N, Morita T, Shimada T: "Telomerase activity and Bcl-s expression in the gallbladder of pancreaticobiliary maljunction-A preliminary study"J Hepatobiliary Pancreat Surg (in press). 10. (2003)

Ichikawa Y、Kamiyama M、Sekido H、Ishikawa T、Miura Y、Kamiya N、Morita T、Shimada T：“胰胆管畸形胆囊中的端粒酶活性和 Bcl-s 表达 - 初步研究”J Hepatobiliary Pancreat Surg（出版中）

Y Koyanagi: "Pancreaticobiliary Maljunction"Igaku Tosho. 413 (2002)

小柳 Y：“胰胆连接不良”伊岳东照。

Ichikawa Y, et al.: "Telomerase Activity and Bcl-2 expression in the Gallbladder of Pancreaticobiliary Maljunction -A Preliminary Study"J Hepatobiliary Pancreat Surg. (in press). (2003)

Ichikawa Y 等人：“胰胆管连接不良胆囊中的端粒酶活性和 Bcl-2 表达 - 初步研究”J Hepatobiliary Pancreat Surg。

Koyanagi Y: "Pancreaticobiliary Maljunction"Igaku Tosho. 413 (2002)

小柳Y：“胰胆连接不良”伊岳东照。