MECHANISM OF CAR CINOGENESIS IN THE BILIATY SYSTEM-MOLECULAR BIOLOGICAL ANALYSIS OF PANCREATICOBILIARY MALJUNCTION USING CDNA MICROARRAY

利用cDNA微阵列技术对胃肠道癌变机制进行全面分析。材料为胰胆异常（PBMJ）患者手术标本胆囊或胆总管癌或非癌粘膜。从这些样品中提取rna。由于RNA的数量相当少，因此使用Amino MEGA Script T7试剂盒按线性扩增法进行扩增。应用该技术，从1.5 ~ 3微克的总RNA中获得了足够的RNA。扩增前后线性关系良好，相关系数为0.7。利用从PBMJ患者手术标本中提取的RNA样本进行基因表达的综合分析。利用cDNA微阵列技术全面检测基因表达谱的变化。比较PBMJ患者结肠腺瘤和胆道粘膜的这些变化，本研究可能有助于阐明胃肠道癌变的机制。

The mechanism of gastrointestinal carcinogenesis was comprehensively analyzed using cDNA micro array. Materials were the cancerous or non-cancerous mucosa at the gallbladders or common bile ducts taken from surgical specimen of the patients with pancreaticobiliary maljunction (PBMJ). RNAs were extracted from these samples. Because the quantity of the RNA was considerably small, amplification was performed according to the linear amplification method using Amino MEGA Script T7 kit. Applying this technique, the enough RNAs were obtained form 1.5 to 3 micrograms of total RNA. The linearity analysis between before and after the amplification was performed and well correlation was confirmed, correlation coefficient 0.7. Comprehensive analyses of gene expressions were performed using RNA samples extracted from the surgical specimens in the patients with PBMJ. The changes of gene expression patterns were comprehensively detected by cDNA micro array. Comparing these changes between colonic adenomas and the biliary mucosae in PBMJ, this study may contribute to elucidating the mechanism of gastrointestinal carcinogenesis.