MECHANISM OF CAR CINOGENESIS IN THE BILIATY SYSTEM-MOLECULAR BIOLOGICAL ANALYSIS OF PANCREATICOBILIARY MALJUNCTION USING CDNA MICROARRAY

利用cDNA微阵列全面分析胃肠道癌变机制。材料是从胰胆管连接不良（PBMJ）患者的手术标本中取出的胆囊或胆总管处的癌性或非癌性粘膜。从这些样品中提取 RNA。由于RNA的量相当小，因此使用Amino MEGA Script T7试剂盒根据线性扩增方法进行扩增。应用该技术，从 1.5 至 3 微克总 RNA 中获得了足够的 RNA。扩增前后线性分析，相关性良好，相关系数0.7。使用从 PBMJ 患者手术标本中提取的 RNA 样本对基因表达进行综合分析。通过cDNA微阵列综合检测基因表达模式的变化。比较PBMJ中结肠腺瘤和胆道粘膜的这些变化，本研究可能有助于阐明胃肠道癌变的机制。

The mechanism of gastrointestinal carcinogenesis was comprehensively analyzed using cDNA micro array. Materials were the cancerous or non-cancerous mucosa at the gallbladders or common bile ducts taken from surgical specimen of the patients with pancreaticobiliary maljunction (PBMJ). RNAs were extracted from these samples. Because the quantity of the RNA was considerably small, amplification was performed according to the linear amplification method using Amino MEGA Script T7 kit. Applying this technique, the enough RNAs were obtained form 1.5 to 3 micrograms of total RNA. The linearity analysis between before and after the amplification was performed and well correlation was confirmed, correlation coefficient 0.7. Comprehensive analyses of gene expressions were performed using RNA samples extracted from the surgical specimens in the patients with PBMJ. The changes of gene expression patterns were comprehensively detected by cDNA micro array. Comparing these changes between colonic adenomas and the biliary mucosae in PBMJ, this study may contribute to elucidating the mechanism of gastrointestinal carcinogenesis.