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Studies of chemoprevention in rats with colorectal cancer caused by 1, 2-dimethylhydrazine

1, 2-二甲基肼所致大鼠结直肠癌的化学预防研究

基本信息

批准号：
12671280
负责人：
IWAMOTO Sueharu
金额：
$ 1.41万
依托单位：
KAWASAKI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671280/
关键词：
Wistar rat 1,2-dimethylhydrazine colorectal cancer model inducted in rats arabinoxylane fumagillol aberrant crypt foci colorectal cancer chemoprevention 1, 2-dimethylhydrazine Wister系ラット

项目摘要

Purpose : We examined the inhibitory effects of arabinoxylane and fumagillol as chemopreventive drugs on colorectal carcinogenesis initiated by 1, 2-dimethylhydrazine (DMH) in Wistar rats. Materials and Methods : Female Wistar rats were obtained at five weeks of age from Japan SLC Inc.. After a minimum quarantine period of one week, DMH was administered to the rats as a carcinogen and simultaneously they received arabinoxylane or fumagillol as chemopreventive drugs. Subcutaneous injection of 20 mg/kg DMH was done once a week from the age of 6 weeks to 16 weeks. After the injection ofDMH, rats receiving only tap water with a control diet were designated as group 1 (non-treated group). Rats injected with DMH who at the same time received arabinoxylane 50mg/kg orally in their drinking water were designated as group 2.Those receiving a subcutaneous injection offumagillol lOmg/kg or 30mg/kg twice a week from the age of 6 weeks to 36 weeks were designated as groups 3 and 4, respectively. The … More se three groups were the treated groups. At 21 weeks, about half of the rats in the four groups were sacrificed and the numbers of lesions with the atypical crypt foci (ACF) of premalignant lesions observed in the total colorectum were counted. At 36 weeks, the remaining rats in the four groups were sacrificed, the numbers of lesions with colorectal cancers and polyps were counted, and the survival rates of the rats in the four groups were calculated. Results : L, The incidences of ACF in groups 2 and 4 were significantly lower than the incidence in group 1 (p<0.05). There was no difference in the incidences ofACF between group 1 and group 3.JLThe incidences of colorectal cancers and polyps decreased in groups 2, 3ーand 4 compared with that in group 1, but there were no statistically significant differences among the four groups, i The survival rates at 36 weeks were 100 per cent in group 2, 80 per cent in group 3, 62 per cent in group 4, and 32 per cent in group 1.There was a significant difference between group 1 and group 2 (p<0.()5). Conclusion : Administration of arabinoxylane significantly decreased the incidences of ACF and was survived the all of the rats until the end of this experiment. The results suggest that arabinoxylane might be a candidate for chemoprevention strategy for colorectal cancer in humans. Less

目的：本研究观察了化学预防药物阿糖胞苷和烟曲霉醇对二甲基肼（DMH）诱发大鼠大肠癌的抑制作用。材料和方法：从日本SLC公司获得5周龄的雌性Wistar大鼠。在至少一周的检疫期后，将DMH作为致癌物给予大鼠，同时它们接受阿拉伯树胶烷或烟曲霉醇作为化学预防药物。6 ~ 16周龄每周皮下注射DMH 20 mg/kg。注射DMH后，只接受自来水和对照饮食的大鼠被指定为第1组（未处理组）。第2组为DMH注射组，同时饮水中口服阿拉伯树胶烷50 mg/kg。第3组和第4组分别从6周龄至36周龄每周2次皮下注射10 mg/kg和30 mg/kg的富马吉洛。的 ...更多信息三组为治疗组。在21周时，四组中约一半的大鼠被处死，并计数在整个结肠直肠中观察到的癌前病变的非典型隐窝病灶（ACF）的病变数量。第36周处死剩余大鼠，计算各组大鼠的生存率。结果：第2、4组ACF发生率明显低于第1组（P<0.05）。与第1组相比，第2、3、4组的大肠癌和大肠息肉的发生率有所下降，但四组之间的差异无统计学意义。第2、3、4组的36周生存率分别为100%、80%、62%，第1组为32%。第1组与第2组比较有显著性差异（p<0. 05）。（）5）.结论：给药阿拉伯树胶烷可显著降低ACF的发生率，所有大鼠均存活至实验结束。结果表明，阿拉伯糖烷可能是人类结直肠癌化学预防策略的候选者。少