Study for the mechanism of the development of co-lateral vein in portal hypertension

5周龄的同基因BL/6小鼠作为Lac-Z转基因小鼠的供体。行骨髓移植。用900cOy全身照射后。OCL4诱导肝纤维化。采用肝细胞生长因子（HGF）基因转染，观察骨髓细胞在肝再生过程中是否能转移到肝脏。我们有以下发现。在小损伤肝再生中，肝细胞或卵圆形细胞不来源于造血干细胞。将造血干细胞注入内皮细胞后，重建肝脏。在本实验中，共侧静脉发育，但未发现造血细胞。门静脉高压症伴侧静脉形成的机制尚不清楚。

Five-week-old syngeneic BL/6 mouse served as donors for Lac-Z transgenic mouse. Bone marrow transplantation was performed.After whole body irradiation with 900cOy. Fibrotic liverwas induced by administration of OCL4. Hepatocyle growth factor(HGF) gene transfer was performed and examined if bone marrow cell would be delivered to liver or not in regeneration of liver.We got the following finds. Hepatocytes or oval cells did not derive from hematopoietic stem cell in hepatic regeneration in a little damaged liver. After hematopoietic stem cell was delivered to the endothelial cell, the liver was reconstructed. In this experiment, the co-lateral vein developed but hematopoietic cell was not found. We could not show the mechanism of the development of co-lateral vein in portal hypertension.