The development of gene therapy using HSV-TK gene for HCC by ultrasonic tomography guiding

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To achieve gene therapy using HSV-TK gene for Hepatocellular carcinoma (HCC) by Ultrasonic tomography guiding, we established the HCC tumor bearing mice and assessed the efficacy of gene expression by direct injection to tumor nodule with laparotomy last year. As we reported last year, tumor nodules were observed in the liver eight weeks after inoculation via spleen. At this point, most of mice died after gene transduction mediated by Hemaggultinating Virus of Japan (HVJ)-liposome and the efficacy of gene transduction was not clarified. We next tried to deliver two kinds of genes (AFP/LacZ or CMV/LacZ) by direct injection six weeks after tumor cell inoculation. Although survival rate was improved compared to eight weeks, halh of mice were still died. LacZ expression driven by AFP promoter was much lower than CMV promoter in tumor that could obtain from survival mice.Ultrasonic tomography (UST) could faintly detect liver tumor in rats liver but it could not detected tumor in mice liver. Thus, UST guiding gene deliverly was not feasible by available UST in animal model.