Genetic informations of gliomas by whole-genome analysis using genome microarray

使用基因组微阵列进行全基因组分析的神经胶质瘤的遗传信息

• 批准号: 12671356
• 负责人: MARUNO Motohiko
• 金额: $ 2.18万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671356/
• 关键词: CGH microarray; gliomas; deletion; EGFR amplification; genetic informations; oncogene; microarray,; CGH,; glioblastoma,

We attempted to define the genetic informations of gliomas using genome microsrray.[Methods] The DNA copy number aberrations (CNAs) were detected by comparative genomic hybridization (CGH) in 52 gliomas including 37 glioblastomas and the observed gains in CNAs were further verified by DNA microarray analysis in 19 glioblastomas.[Results] CNAs were detectable in all of the 37 samples analyzed and were mainly deletions and frequently involved Chromosomes lp (in 35% instances), 10 (54%), 19q (38%), and 22q (49%.). Gains in CNAs were frequently involved Chromosomes 7 (16%) and 8q (11%).Genomic aberrations in human glioblastomas were deletions mainly and a few amplifications which were seen frequently in de novo type. In 19 cases DNA microarray analysis was performed against 58 oncogenes (AmpliOne I) using GenoSensor System (Vysis, Inc., Downers Grove, IL, USA). Amplification of EGFR (7pl2) were seen in 6 cases (31%), PDGFRA (4ql2) in 2 case (11%), MYC(8ql2) in 1 case (5%), and CCND/FGF4 (1 lq3) in one case. These amplifications were relatively strong ranged from 4 times to 32 times. The date about chromosome 7 from CGH and genome microarray was inconincidence. [Conclusion] It is apparent to get genetic informations casily and rapidly from clinical samples using microarray system It must be used not only in diagnosis but in order made therapy.

[方法]采用比较基因组杂交技术(CGH)检测52例胶质瘤(包括37例胶质母细胞瘤)的DNA拷贝数畸变率(CNA)，并对19例胶质母细胞瘤进行DNA微阵列分析。[结果]37例胶质母细胞瘤标本中均可检测到CNA，主要为缺失和频繁受累的染色体LP(35%)、10(54%)、19q(38%)和22q(49%)。在人脑胶质母细胞瘤中，基因组异常以缺失和少量扩增为主，常见于从头类型。在19个病例中，使用基因传感器系统(美国伊利诺伊州唐纳斯格罗夫的Vosis，Inc.)对58个癌基因(AmpliOne I)进行DNA微阵列分析。EGFR(7pl2)扩增6例(31%)，PDGFRA(4q12)扩增2例(11%)，MYC(8q12)扩增1例(5%)，CCND/FGF4(1q3)扩增1例。扩增倍数从4倍到32倍不等。CGH和基因组芯片测得的7号染色体的数据不一致。[结论]利用基因芯片系统可以方便、快速地从临床标本中获取遗传信息，不仅可用于诊断，还可用于临床治疗。

项目成果

Jamshidi J, et al.: "Central neurocytoma presenting with intratumoral hemorrhage. a case report"Neurosurg Res. 24. 48-52 (2001)

Jamshidi J 等人：“伴有瘤内出血的中枢神经细胞瘤。病例报告”神经外科研究。

Kohmura E, et al.: "Usefulness of synaptophysin immunohistochemstry in an adult case of chroid plexus carcinoma"Neurol Res. 22. 478-480 (2000)

Kohmura E 等人：“突触素免疫组织化学在成人脉络丛癌病例中的作用”Neurol Res。

Maruno M, et al.: "Profile of genetic aberrations in human glioblastomas : CGH and genomic microarray study"Proceedings of Ihe American Association for Cancer Research. 42. 654 (2001)

Maruno M 等人：“人类胶质母细胞瘤遗传畸变概况：CGH 和基因组微阵列研究”美国癌症研究协会会议录。

Izumoto S, et al.: "PTEN mutaions in malignant gliomas and their relation with meningeal careinomatosis"J Neurooncol. 53. 21-26 (2001)

Izumoto S 等人：“恶性神经胶质瘤中的 PTEN 突变及其与脑膜癌瘤病的关系”J Neurooncol。

Maruno M, et al.: "Whole-genome analysis of human astrocytic tumors by comparative genomic hybridization"Brain Tumor Pathol. 17. 21-27 (2000)

Maruno M 等人：“通过比较基因组杂交对人类星形细胞肿瘤进行全基因组分析”脑肿瘤病理学。