Genetic aberrations in gliomas detected by comparative genomic hybridization (CGH)

比较基因组杂交（CGH）是一种分子细胞遗传学技术，它可以在一次实验中筛查整个染色体区域的丢失和获得。我们研究了34例不同组织学恶性肿瘤的星形细胞肿瘤的染色体畸变（星形细胞瘤7例，间变性星形细胞瘤3例，和胶质母细胞瘤24）的比较基因组杂交（CGH）结果表明，染色体丢失区数与组织学恶性程度呈负相关，1p（41%）、10（29%）、19q（44%）和22q（38%）是最常见的4种染色体畸变，胶质母细胞瘤多染色体畸变率高于星形细胞瘤和间变性星形细胞瘤（p<0.001）。原发性胶质母细胞瘤具有高频率的染色体获得，而来自间变性星形细胞瘤的复发性胶质母细胞瘤则没有。这项技术似乎是一种强有力的工具，在调查肿瘤DNA的改变和表征胶质母细胞瘤中，星形细胞系肿瘤生物学。

Comparative genomic hybridization (CGH) is a molecular cytogenetic technique that allows screening for losses and gains of entire chromosomal regions in a single experiment.We investigated chromosomal aberrations in 34 astrocytic tumors of various histologic malignancies (astrocytoma 7, anaplastic astrocytoma 3, and glioblastoma 24) by comparative genomic hybridization (CGH).The results demonstrated that the number of areas of chromosomal loss was negatively correlated with histologic malignancies.These losses of portions of chromosomes on 1p (41%), 10 (29%), 19q (44%), and 22q (38%) were the four most frequent chromosomal aberrations.The aberrations were observed in multiple chromosomes more frequently in glioblastomas than in astrocytomas and anaplastic astrocytomas (p<O.OOl).Primary glioblastomas have a high frequency of chromosomal gains, whereas recurrent glioblastomas from anaplastic astrocytomas do not.This technique seems to be a powerful tool in surveying the tumor DNA alterations and characterizing the biology of tumors of astrocytic lineage.