Genetic aberrations in gliomas detected by comparative genomic hybridization (CGH)

通过比较基因组杂交（CGH）检测神经胶质瘤的遗传畸变

• 批准号: 09671423
• 负责人: MARUNO Motohiko
• 金额: $ 1.98万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671423/
• 关键词: gliomas; CGH; Loss of 1p, 10,19q, and 22q; gain; hitological malignancy; malignant transformation; 染色体欠失・増幅; 第1・19・22番染色体の欠失; 初発膠芽腫; 生存期間

Comparative genomic hybridization (CGH) is a molecular cytogenetic technique that allows screening for losses and gains of entire chromosomal regions in a single experiment.We investigated chromosomal aberrations in 34 astrocytic tumors of various histologic malignancies (astrocytoma 7, anaplastic astrocytoma 3, and glioblastoma 24) by comparative genomic hybridization (CGH).The results demonstrated that the number of areas of chromosomal loss was negatively correlated with histologic malignancies.These losses of portions of chromosomes on 1p (41%), 10 (29%), 19q (44%), and 22q (38%) were the four most frequent chromosomal aberrations.The aberrations were observed in multiple chromosomes more frequently in glioblastomas than in astrocytomas and anaplastic astrocytomas (p<O.OOl).Primary glioblastomas have a high frequency of chromosomal gains, whereas recurrent glioblastomas from anaplastic astrocytomas do not.This technique seems to be a powerful tool in surveying the tumor DNA alterations and characterizing the biology of tumors of astrocytic lineage.

比较基因组杂交(CGH)是一种分子细胞遗传学技术，可以在单个实验中筛选整个染色体区域的丢失和获得。我们用比较基因组杂交(CGH)技术研究了34例星形细胞肿瘤(星形细胞瘤7例、间变性星形细胞瘤3例和胶质母细胞瘤24例)的染色体畸变率。结果表明，染色体丢失的面积与组织学恶性肿瘤呈负相关。这些染色体丢失的部分1p(41%)，10(29%)，19q(44%)，与星形细胞瘤和间变性星形细胞瘤相比，胶质母细胞瘤在多条染色体上的异常发生率更高(p&lt；原发胶质母细胞瘤有很高的染色体获得率，而间变性星形细胞瘤复发的胶质母细胞瘤则没有。这项技术似乎是研究肿瘤DNA变化和表征星形细胞肿瘤生物学特性的有力工具。

项目成果

Maruno M,et al.: "Chromosomal aberrations detected by comparative genomic hybridization(CGH)in astrocytic tumors." Cancer Lett. 135. 61-66 (1999)

Maruno M 等人：“通过比较基因组杂交 (CGH) 检测星形细胞肿瘤中的染色体畸变。”

Muhammad AKMG, et al.: "Topical application of fibrin adhensive in the rat brain : Effects on different cellular elements of the wound." Neurol Res. 19. 84-88 (1997)

Muhammad AKMG 等人：“在大鼠大脑中局部应用纤维蛋白粘合剂：对伤口不同细胞成分的影响。”

Maruno M,et al.: "Chromosomal losses and gains in meningiomas : Comparative genomic hybridization (CGH) study of the whole genome." Neurol Res. 20. 612-616 (1998)

Maruno M 等人：“脑膜瘤中的染色体丢失和增益：全基因组的比较基因组杂交 (CGH) 研究。”

Muhammad AKMG, et al.: "Cromosome 22q allelic losses of microsatelliteloci in human astrocytic tumors and its clinical relevance." Neurol Med Chir (Tokyo). 37. 606-611 (1997)

Muhammad AKMG 等人：“人类星形细胞肿瘤中微卫星的染色体 22q 等位基因丢失及其临床相关性。”

Muhammad AKMG,Yoshimine T,Maruno M,Tokiyoshi K,Isaka T,Takemoto O,Hayakawa T: "Cromosome 22q allelic losses of microsatelliteloci in human astrocytic tumors and its clinical relevance." Neurol Med Chir (Tokyo). 37. 606-611 (1997)

Muhammad AKMG、Yoshimine T、Maruno M、Tokiyoshi K、Isaka T、Takemoto O、Hayakawa T：“人类星形细胞肿瘤中微卫星的染色体 22q 等位基因丢失及其临床相关性。”