Function of Prostaglandin D Synthase in Cerebrospinal Fluid after Subarachnoid Hemorrhage / Application for the Treatment of Cerebral Vasospasm

点击翻译按钮获取中文摘要

In order to test the hypothesis that lipocalin-type prostaglandin D synthase (PGDS) in cerebrospinal fluid (CSF) may trap or scavenge substances which cause cerevral vasospasm after subarachnoid (SAH) hemorrhage and reduce ischemic sequela, we performed experimental and clinical evaluations and obtained following results.(1) The PGDS level in lumbar CSF increased about 2-fold at day 3 and day 5. The transient and delayed increase in the PGDS level in CSF is due to its induction of PGDS in the arachnoid membrane after SAH.(2) The substance binding to PGDS in CSF of patients with SAH was shown to be biliverdin by GC mass spectroscopy.(3) Human recombinant PGDS injected into the subarachonoid space of dog was tranported to blood quickly and finall discharged into urine. PGDS injected into blood never went into CSF.(4) PGDS binding biliverdin was shown in urine of patients with SAH.(5) Biliverdin oxides was one of strong vasoconstrictors in vitro.(6) There was a tendency that intrathecal injection of PGDS might reduce the severity of vasospasm estimated by angiography using couble-injection SAH canine model.