The potential storage of peripheral nerve grafts using polyphenol

使用多酚储存周围神经移植物的潜力

• 批准号: 12671412
• 负责人: KAKINOKI Ryosuke
• 金额: $ 2.18万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671412/
• 关键词: Polyphenol; storage of peripheral nerve grafts; peripheral nerve regeneration; 神経保存; 末梢神経再生

The potential storage of peripheral nerve grafts using polyphenol was assessed in a rat sciatic nerve model. Isogenic sciatic nerve grafts 20 mm in length were stored either in polyphenol or Dulbecco's Modified Eagle Medium (DMEM) prior to transplantation. Isografting of fresh nerves was also done. Graft cells were kept more viable in the polyphenol-treated nerve segments than in the nerves stored in culture medium. The histological and electrophysiological outcomes of nerve regeneration through polyphenol-treated nerves was significantly better than those through nerves stored in DME medium and almost equal to those through fresh nerve grafts at 24 weeks postengraftment,. This study shows the potential ability of tissue preservation using polyphenol.

在大鼠坐骨神经模型中评估使用多酚的周围神经移植物的潜在储存。在移植前，将20 mm长的同基因坐骨神经移植物储存在多酚或Dulbecco改良Eagle培养基（DMEM）中。新鲜神经也进行了同种异体移植。移植细胞在多酚处理的神经节段中比在培养基中储存的神经中保持更有活力。术后24周，经多酚处理的神经再生的组织学和电生理结果明显好于经DME保存的神经，与新鲜神经移植的结果基本相当。这项研究显示了使用多酚的组织保存的潜在能力。