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Developing the therapeutic methods for mitochondrial function, which may related to the neuronal recovery in penumbral region

开发线粒体功能的治疗方法，这可能与半暗区神经元的恢复有关

基本信息

批准号：
12671470
负责人：
NAGANO Osamu
金额：
$ 1.92万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671470/
关键词：
Mitochondria Penumbra NADH 部分脳虚血 NADH蛍光アポトーシス DC-ポテンシャル

项目摘要

To elucidate the propagation of recurrent depolarization in the penumbra, the fluorescence of cortical NADH was observed in rats that had been subjected to focal ischemia. Ischemia was initiated by tightening the left CCA ligature and occluding the MCA by lifting the vessel 1 mm above the cortical surface with an 80-μm stainless-steel hook attached to a micromanipulator. A large cranial window was made on the left parietal-temporal bone to observe almost the entire left cortical hemisphere. The cortical surface was illuminated using a UV lamp (366nm), and images of NADH fluorescence were taken every 17 seconds using a CCD camera with a 460nm bandpass filter. During a three-hour period of ischemia, 220 recurrent depolarizations were observed in 45 animals as waves of high signal intensity that originated at the edge of the ischemic core and moved along the round margin of the ischemic core. Recurrent depolarizations were classified into two types : "spread-out type (86%)" in which recurrent depolarization spread out and did not increase the area of the ischemic core, and "expansion type (14%)" in which recurrent depolarization merged with the ischemic core and increased the area of the ischemic core. Histological examination revealed that neuronal injury occurred in the penumbral region, in which "spread-out type" of recurrent depolarization was observed, and that infarction occurred in the ischemic core, in which "expansion type" of recurrent depolarization was observed.

为了阐明在半影区复发性去极化的传播，观察了局灶性缺血大鼠皮质NADH的荧光。通过收紧左侧CCA结扎线并通过用连接到显微操作器上的80 μm不锈钢钩将血管提升到皮质表面上方1 mm来闭塞MCA来引发缺血。在左侧顶颞骨上制作一个大的颅骨窗，以观察几乎整个左侧皮质半球。使用UV灯（366nm）照射皮质表面，并且使用具有460nm带通滤波器的CCD照相机每17秒拍摄NADH荧光的图像。在3小时的缺血期间，在45只动物中观察到220次复发性去极化，其为起源于缺血核心边缘并沿着缺血核心圆形边缘移动的高信号强度波。复发性去极化分为两种类型：“扩散型（86%）”，其中复发性去极化扩散，不增加缺血核心的面积，和“扩张型（14%）”，其中复发性去极化合并与缺血核心，增加缺血核心的面积。组织学检查显示，神经元损伤发生在半影区，其中观察到的“扩散型”的再去极化，和梗死发生在缺血的核心，其中观察到的“扩张型”的再去极化。