Inhalation anesthetics suppress c-Fos expression evoked by noxious somatic stimulation in rat spinal cord dorsal horn neurons

吸入麻醉药抑制大鼠脊髓背角神经元中有害体细胞刺激引起的 c-Fos 表达

• 批准号: 12671492
• 负责人: FUKUDA Yusaku
• 金额: $ 1.47万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671492/
• 关键词: Anesthetics; Enflurane; Isoflurane; Sevoflurane; Noxious stimulation; Formalin; Spinal cord dorsal horn; c-Fos; Fos蛋白

The effects of inhalation anesthetics, enflurane, isoflurane, and sevoflurane, on the expression of c-Fos protein evoked by formalin injection were studied in the spinal cord in the rat. Male Sprague-Dawley rats were allocated to four treatment groups: 100% oxygen (control, n=6), 3.3% enflurane (1.5MAC, N=6), 2.1% isoflurane (1.5MAC, N=6)4.0%, sevoflurane (1.5 MAC, n=6) for 30 nun. Each rat then received a s.c. inject of 5/o formahn 100μ1 into the left hind paw and anesthesia was maintained. Three hours later the rats were sacrificed and perfused. Section of the Ll〜6 level of spinal cord were immunostained with anti c-Fos antibody. We counted the number of Fos-like immunoreactive (FLI) cells in every specific lamina.In the sections of L3 and L4 level, the numbers of FLI labeled neurons for groups given enflurane, isoflurane, and sevoflurane were 40 to 50 % less than the control (p<0.05). The decrease occurred predominantly in the deeper layer of the dorsal horn (laminae III〜VI) and ventral gray (laminae VII〜X) but not on the neurons existed in superficial layer (laminae I〜II).This finding was consistent with the results of the previous studies concerned with halothane. These suggested that most inhalation anesthetics have effects on spinal neurons in the deeper layers, some of which directly receive noxious inputs. Our study failed to confirm previous findings that enflurane and sevoflurane augment the afferent signals evoked by the noxious stimulation.

研究了吸入麻醉剂安氟醚、异氟醚和七氟醚对注射福尔马林引起的大鼠脊髓c-Fos蛋白表达的影响。雄性Sprague-Dawley大鼠30只，分为100%氧（对照，n=6）、3.3%安氟醚（1.5MAC, n=6）、2.1%异氟醚（1.5MAC, n=6）、4.0%七氟醚（1.5MAC, n=6） 4组。每只大鼠左后爪皮下注射5/o量的福尔曼100μ1，维持麻醉。3小时后处死大鼠并灌注。用抗c-Fos抗体对脊髓Ll ~ 6层切片进行免疫染色。我们统计了每个特定层中fos样免疫反应（FLI）细胞的数量。在L3和L4水平切片上，安氟醚组、异氟醚组和七氟醚组FLI标记神经元数量比对照组减少40 ~ 50% （p<0.05）。神经元数量减少主要发生在背角较深层（III ~ VI层）和腹侧灰质（VII ~ X层），而浅层（I ~ II层）神经元数量减少不明显。这一发现与先前有关氟烷的研究结果一致。这些结果表明，大多数吸入麻醉剂对更深层的脊髓神经元有影响，其中一些直接接受有害输入。本研究未能证实先前的发现，即安氟醚和七氟醚增强了由有害刺激引起的传入信号。

项目成果

深田 祐作, 他: "吸入麻酔薬ハロタンの脊髄レベルにおける麻酔作用の機序-fos蛋白発現を指標とした検討-"麻酔. 48. 966-976 (1999)

Yusaku Fukada 等：“吸入麻醉剂氟烷在脊髓水平的麻醉作用机制 - 使用 fos 蛋白表达作为指标的研究”麻醉 48. 966-976 (1999)。

Fukada Y, et al.: "The study of the anesthetic action of halothane on the rat spinal cord by Fos immunoreactivity"Masui. 48. 966-976 (1999)

Fukada Y 等人：“通过 Fos 免疫反应性研究氟烷对大鼠脊髓的麻醉作用”Masui。

深田 祐作, 他: "吸入麻酔薬ハロタンの脊髄レベルにおける麻酔作用の機序"麻酔. 48・9. 966-976 (1999)

Yusaku Fukada 等：“吸入麻醉剂氟烷在脊髓水平的麻醉作用机制”麻醉 48・9（1999）。