Basic research of the gene therapy of testicular tumor cell

睾丸肿瘤细胞基因治疗的基础研究

• 批准号: 12671537
• 负责人: KAMANO Hiroshi
• 金额: $ 2.11万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671537/
• 关键词: A-myb; B-myb

The myb family consists of three members, the c-myb protooncogene and myb related genes A-myb and B-myb, all of which encode nuclear sequence-specific DNA-binding protein. C-myb proteins is expressed at high levels in most hematopoietic precursor cells and plays a crucial role in the proliferation and differentiation of these cells.In the presence of DNA antisense B-myb oligo the proliferation rate of cell was significantly decreased on the fourth day. In the presence of mismatched DNA antisense B-myb oligo the proliferation 'rate of cell was not decreased. In the other hand, in the presence of morpholino antisense B-myb oligo the proliferation rate of cell was significantly decreased. In the presence of mismatched morpholin antisense B-myb oligo the proliferation rate of cell was not decreased.

myb家族由c-myb原癌基因、myb相关基因A-myb和B-myb组成，它们都编码核序列特异性DNA结合蛋白。C-myb蛋白在大多数造血前体细胞中高水平表达，在造血前体细胞的增殖和分化中起重要作用，反义B-myb寡核苷酸作用于造血前体细胞后，第4天细胞增殖率明显降低。错配DNA反义B-myb寡核苷酸对细胞增殖无抑制作用。而在反义寡核苷酸的存在下，细胞的增殖率明显降低。在反义寡核苷酸存在下，细胞增殖率不受影响。

项目成果

Okutani Y, Kitanaka A, Tanaka T, Kamano H: "Src directly tyrosine-phosphorylates STAT5 on its activation site and is involved in erythropoietin-induced signaling pathway"oncogene. 20(45). 6643-6650 (2001)

Okutani Y、Kitanaka A、Tanaka T、Kamano H：“Src 在其激活位点直接酪氨酸磷酸化 STAT5，并参与促红细胞生成素诱导的信号传导途径”癌基因。

Kubota Y, Tanaka T, Kitanaka A, Ohnishi H, Okutani Y, Waki M, Ishida T, Kamano H: "Src transduces erythropoietin-induced differentiation signals through phosphatidylinositd 3-kinase"EMBOJ. 20(20). 5666-5677 (2001)

Kubota Y、Tanaka T、Kitanaka A、Ohnishi H、Okutani Y、Waki M、Ishida T、Kamano H：“Src 通过磷脂酰肌醇 3-激酶转导促红细胞生成素诱导的分化信号”EMBOJ。

Kamano H, Sizmann J, Trauth K et al.: "Functional analysis of B-myb gene Expression of B-myb, alternative Splicing of B-myb gene(exon9a), and heat shock element dependent trans activation of the human HSP70 promotor"The First Waterfront Symposium of Human

Kamano H、Sizmann J、Trauth K 等人：“B-myb 基因的功能分析、B-myb 基因的表达、B-myb 基因（外显子 9a）的选择性剪接以及人类 HSP70 启动子的热休克元件依赖性反式激活”

Kamano H: "Photodynamic effects of gallium-metal porphyrin on human leukemia cells in combination with high-brightness LED light"Transplant.Proc.. 32(7). 2442-2443 (2000)

Kamano H：“镓金属卟啉与高亮度 LED 灯结合对人类白血病细胞的光动力效应”Transplant.Proc.. 32(7)。