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The relationship between the effects of free fatty acid on the expression of gene encoding carbohydrate metabolism and insulin resistance during pregnancy

妊娠期游离脂肪酸对碳水化合物代谢基因表达的影响与胰岛素抵抗的关系

基本信息

批准号：
12671592
负责人：
KUSAKA Hideto
金额：
$ 0.7万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Dramatic alternations in maternal metabolism occur during gestation and lactation, especially glucose and fat metabolism. For example, in rats, the amount of body fat mass increases during gestation, then decreases just prior to delivery, and remains low after parturition. To investigate the factors involved in such changes in maternal fat mass, messenger ribonucleic acid (mRNA) levels of adipocytokines, peroxisome proliferator-activated receptor-γ (PPAR-γ) and tumor necrosis factor-α (TNF-α), were examined in the intraabdominal adipose tissue of non-pregnant rats, pregnant rats and postpartum rats. We also examined the issue of whether apoptosis, which could be promoted by PPAR-γ and TNF-α, is involved in any of the changes in maternal fat mass. The activity of lipoprotein lipase (LPL) and hormone sensitive lipase (HSL) in adipose tissue was also measured. PPAR-γ and TNF-α mRNA levels remained constant during the gestational and postpartum periods. Apoptosis was not detected at any time as evidenced by DNA laddering and in situ staining. LPL activity was significantly increased at day 5 and remained elevated until day 14 of gestation. HSL activity was significantly increased at day 10 of gestation and then decreased after delivery, at day 10 of lactation.In conclusion, during the gestational and postpartum period of rats, changes in maternal fat mass did not directly correlate with the levels of expression of PPAR-γ and TNF-α mRNA. Apoptosis also does not appear to influence on fat mass change. The changes in LPL and HSL activities during gestation suggest that these enzymes might be regulators of maternal adipose tissue level.

妊娠和哺乳期间，母体代谢发生显着变化，尤其是葡萄糖和脂肪代谢。例如，在大鼠中，体脂量在妊娠期间增加，然后在分娩前减少，并在分娩后保持较低水平。为了研究母体脂肪量变化的相关因素，我们检测了非妊娠大鼠、妊娠大鼠和产后大鼠腹内脂肪组织中脂肪细胞因子、过氧化物酶体增殖物激活受体-γ（PPAR-γ）和肿瘤坏死因子-α（TNF-α）的信使核糖核酸（mRNA）水平。我们还研究了 PPAR-γ 和 TNF-α 促进的细胞凋亡是否与母体脂肪量的任何变化有关。还测量了脂肪组织中脂蛋白脂肪酶（LPL）和激素敏感脂肪酶（HSL）的活性。 PPAR-γ 和 TNF-α mRNA 水平在妊娠期和产后期间保持恒定。 DNA梯状图和原位染色证明任何时候都没有检测到细胞凋亡。 LPL 活性在妊娠第 5 天显着增加，并保持较高水平直至妊娠第 14 天。 HSL活性在妊娠第10天显着升高，而在分娩后、哺乳第10天则降低。综上所述，在大鼠妊娠期和产后期，母体脂肪量的变化与PPAR-γ和TNF-α mRNA的表达水平没有直接相关。细胞凋亡似乎也不影响脂肪量的变化。妊娠期间 LPL 和 HSL 活性的变化表明这些酶可能是母体脂肪组织水平的调节剂。