The basic study concerning the high pregnancy rate by in vitro fertilization-embryo transfer treatment in infertile women with antisperm antibody

CBA/J雌性小鼠与C57 BL/6雄性小鼠交配。在阴道涂片中检测到精子存在的时间指定为妊娠第0.5天。从非妊娠和妊娠小鼠中切除子宫。在消化污染DNA后，分离总RNA并进行逆转录。采用TaqMan化学定量PCR方法检测降钙素mRNA的表达，以甘油醛-3-磷酸脱氢酶（GAPDH）mRNA的表达水平为内标（0.71 +/- 0.31）和2.5（22.55 +/- 10.69），在妊娠第3.5天达到峰值（111.38 +/- 120.92），并在第4.5天降至21.54 +/- 3.65。降钙素mRNA在妊娠第7.5天（21.12 +/- 24.19）和第9.5天（32.73 +/- 3.12）继续表达。然而，在妊娠第9.5天，未出现胚胎的子宫部分中降钙素mRNA/GAPDH mRNA的比值非常低（2.71 +/- 1.65）。此外，降钙素mRNA在妊娠第7.5天和第9.5天表达的结果表明，降钙素mRNA的表达也受到胚胎信号的调节。

CBA/J female mice were mated with C57BL/6 male mice. The time when the presence of sperm was detected in a vaginal smear was designated day 0.5 of gestation. The uteri were excised from nonpregnant and pregnant mice. Total RNA was isolated and reverse-transcribed after the digestion of contaminating DNA. Quantitative PCR for calcitonin mRNA was performed by using Taq Man Chemistry, and the expression level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was measured as an internal standard.The ratio of calcitonin mRNA/GAPDH mRNA in the uterus dramatically increased between days 1.5 (0.71 +/- 0.31) and 2.5 (22.55 +/- 10.69) of gestation, reached a peak on day 3.5 (111.38 +/- 120.92), and decreased to 21.54 +/- 3.65 on day 4.5. Calcitonin mRNA continued to be expressed on day 7.5 (21.12 +/- 24.19) and day 9.5 (32.73 +/- 3.12) of gestation. However, the ratio of calcitonin mRNA/GAPDH mRNA in the part of the uterus in which the embryo was not present was very low on day 9.5 (2.71 +/- 1.65) of gestation.These findings demonstrate that calcitonin mRNA in the uteri of mice appeared to be expressed during the implantation period. Furthermore, the findings that calcitonin mRNA was expressed on day 7.5 and day 9.5 of gestation suggest that calcitonin mRNA expression is also regulated by the embryonal signal.