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Significance of Interleukin Converting Enzyme/Caspase-1 in Endometrium and Decidua

白细胞介素转换酶/Caspase-1在子宫内膜和蜕膜中的意义

基本信息

批准号：
12671626
负责人：
ISHIHARA Osamu
金额：
$ 1.98万
依托单位：
Saitama Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

The control of arachidonic acid metabolites production, such as prostaglandin (PG) production, has been described to act major roles in various aspects of reproductive physiology, i.e., implantation of embryo, maintenance of pregnancy, labor and delivery. Since the inducible type of cyclooxygenase (COX-2), the key enzyme of PG production, is induced by interleukin-1 (IL-1) in decidua and endometrium, the present study aimed the significance of interleukin-1 converting enzyme/Caspase-1 in these tissues.As the results, decidual tissues showed the presence of interleukin-1 converting enzyme/caspase-1 immunohistochemically which suggested the possible involvement of the enzyme in transformation of pro IL-1 to IL-1 in decidua. However, there was no significant variance between the samples collected at the times with and without labor.On the other hand, we investigated recurrent implantation failure after embryo transfer in our IVF/ICSI program. Within these cases, through laboratory tests revealed the presence of the cases with Sjogren syndrome, that is well-known syndrome characteristic of apoptosis in exocrine glands of whole body, implying the possible involvement of Caspase system and Fas/FasL system. Since these cases are effectively treated with the administration with predonisolone, we might have found an appropriate models for the investigation for implantation failure in terms of local regulation of PG production in endometrium.

花生四烯酸代谢物的产生，如前列腺素（PG）的产生，在生殖生理的各个方面起着重要的作用，如胚胎的着床、妊娠的维持、分娩和分娩。由于在蜕膜和子宫内膜中，白细胞介素-1 （IL-1）诱导型环氧化酶（COX-2）是PG生成的关键酶，本研究旨在探讨白细胞介素-1转换酶/Caspase-1在这些组织中的意义。结果显示蜕膜组织中存在白细胞介素-1转化酶/caspase-1，提示该酶可能参与蜕膜中pro IL-1向IL-1的转化。然而，在有劳动和没有劳动的时间收集的样本之间没有显着差异。另一方面，我们研究了IVF/ICSI项目中胚胎移植后复发性植入失败。在这些病例中，通过实验室检测发现存在干燥综合征，这是众所周知的全身外分泌腺细胞凋亡的综合征特征，提示可能涉及Caspase系统和Fas/FasL系统。由于这些病例通过强的松龙治疗有效，我们可能已经找到了一种合适的模型来研究子宫内膜中PG生成的局部调节。