EXPLOITATION OF NEW DIAGNO-THERAPEUTIC PROCEDURES OF HUMAN ENDOCERVICAL ADENOCARCINOMA OF THE UTERUS BASED ON CYTOGENETIC MECHANISMS OF MULTI- STEP CARCINOGENESIS

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We analysed the mutational status of the TGF β RII, BAX, and PTEN/MMAC1 genes as well as microsatellite instability (MI) in consecutive cases of endocervical adenocarcinoma of the uterus operated on at our Institute. To examine copy number losses and/or amplifications at the chromosomal region bearing all the genes, we used comparative genornic hybridization (CGH) analysis. CGH analysis may provides a genome-wide overview about tumor-associated genomic imbalances. Among the tumors, a few showed, the MI+ phenotype. Also, few tumors demonstrated a significant mutation with a definite amino acid change in PTEN/MMAC1 gene. CGH analysis demonstrated that a few tumors showed chromosomal copy number losses and/or amplifications, whereas there are no common chromosomal foci showing losses or amplifications among the tumors analysed so far. The role of TGF β RI and BAX genes may be limited as a target gene of MI+ phenotype in endocervical adenocarcinoma, because only a few tumors demonstrated MI+ phenotype in these tumors.Additional explorations are needed to elucidate the cytogenetic mechanisms of multistep carcinogenesis in endocervical adenocarcinomas of the uterus.