Apoptosis-promoting gene Bax transfer potentiates themosensitivity of squamous cell caruroma I vitro

促凋亡基因 Bax 转移增强鳞状细胞癌的热敏感性 I 体外

• 批准号: 12671656
• 负责人: OHTSUBO Toshio
• 金额: $ 2.56万
• 依托单位: Fukui Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671656/
• 关键词: Bax; Hyperthermia; Apoptosis; bax; アポトーシス; ハイパーサーミア; 頭頚部腫瘍; 低pH

To investigate the role of bax gene expression in modulating thermosensitivity through an apoptosis-related mechanism, we examined the thermosensitivity and apoptosis after heating in a human head and neck cancer cells (IMC-3 cell lines) transfected with bax gene-bearing mammalian expression vector. Cellular thermosensitivity was determined using the clonogenic assay. Apoptosis was assessed on the basis of the occurrence of apoptotic cells, DNA fragmentation and poly(ADP-ribose)polymerase (PARP) cleavage. Overexpression of the bax gene in IMC-3 cells enhanced thermosensitivity compared with parent cells. The induction of apoptotic cells, DNA fragmentation and PARP cleavage in bax transfected IMC-3 cells were more prominent than those in IMC-3 cells. These findings suggest that bax contributes to thermosensitization by enhancing the induction of haat-induced apoptosis.

为探讨bax基因表达在调节肿瘤细胞热敏感性中的作用，我们研究了bax基因哺乳动物表达载体转染人头颈癌细胞（IMC-3细胞系）后，对肿瘤细胞热敏感性和热诱导凋亡的影响。使用克隆形成试验测定细胞的热敏感性。根据凋亡细胞的发生、DNA片段化和聚（ADP-核糖）聚合酶（PARP）裂解评估凋亡。与亲本细胞相比，过表达bax基因的IMC-3细胞中的温度敏感性增强。bax基因转染的IMC-3细胞较未转染的IMC-3细胞凋亡明显，DNA断裂和PARP裂解明显。这些发现表明bax通过增强haat诱导的细胞凋亡的诱导作用而促进了热敏感性。

项目成果

Ohtsubo T: "Acidic environment modifies heat -or radiation- induced apoptosis in human waxillary cancer cells."Int.J.Radiation Oncol.Biol.Phys.. (in press). (2001)

Ohtsubo T：“酸性环境改变了人类蜡状癌细胞中热或辐射诱导的细胞凋亡。”Int.J.Radiation Oncol.Biol.Phys..（正在出版）。

Ohtsubo T et al.: "Enhancement of cell killing by induction of apoptosis after treatment with mild hypothermia at 42℃ and cisplatin"Radiation Res. 156. 103-109 (2001)

Ohtsubo T 等人：“42℃轻度低温和顺铂处理后通过诱导细胞凋亡增强细胞杀伤”Radiation Res. 156. 103-109 (2001)

Ohtsubo T. et al.: "Acidic environment modifies heat-or radiation-induced apoptosis in human maxillary cancer cells"Int J Radiation Oncol Biol. Phys. 49. 1391-1398 (2001)

Ohtsubo T. 等人：“酸性环境改变了人类上颌癌细胞中热或辐射诱导的细胞凋亡”Int J Radiation Oncol Biol。

Ohtsubo Y, igawa H, Saito H, Matsumoto H, Park HJ, Song cw kano E, Saito H.: "Acidic environment modifies heat- or radiation- imdicced apoptosisin human warillary cancer cells."Int J Radiation Oncol Biol Phys. 49. 1391-1398 (2001)

Ohtsubo Y、ikawa H、Saito H、Matsumoto H、Park HJ、Song CW kano E、Saito H.：“酸性环境会改变人类战争癌细胞中的热或辐射诱导的细胞凋亡。”Int J Radiation Oncol Biol Phys。

Ohtsubo T et al.: "Acidic environment modifies heat-or radiation-induced apoptosis in human maxillary cancer cells"Int J Radiation Oncol Biol Phys. 49. 1391-1398 (2001)

Ohtsubo T 等人：“酸性环境改变了人类上颌癌细胞中热或辐射诱导的细胞凋亡”Int J Radiation Oncol Biol Phys。