The Vestibular Ocular Reflex of the mutant mice deficient in delta 2 Glutamate receptor Subunit after unilateral labyrinthectomy

单侧迷路切除术后δ2谷氨酸受体亚基缺陷突变小鼠的前庭眼反射

基本信息

批准号：
12671662
负责人：
TSUJI Jun
金额：
$ 2.11万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671662/
关键词：
delta-2 subunit of glutamate receptor vestibular compensation unilateral labyrinthectomy vestibulo ocular reflex c-Fos medial vestibular nucleus inferior vestibular nicleus prepositus hypoglossal nucleus 前庭眼反射グルタミン酸受容体δ2サブユニット赤外線前

项目摘要

Ionotrophic delta-2 subunit of glutamate receptor is specifically expressed only in cerebellar Purkinje cells in the nervous system, and mutant mice deficient in delta 2 proteins have been raised (Kashiwabuchi 1995).These mice did nor exhibit long term depression (LTD), which is one form of synaptic plasticity (Hirano 1995). We examined the postural righting reflex and vestibulo ocular reflex (VOR) after unilateral labyrinthectomy (UL) in these mutant mice, to investigate the involvement of LTD in vestibular compensation. And we also examined the c-Fos expression after unilateral ladyrinthectomy in these mutant mice, to investigate the morphological change during the vestibular compensation.After UL in the knock out mice deficient in delta 2 glutamate receptor subunit, the recovery of the postural righting reflex and VOR are retarded than normal mice, which suggests delta 2 glutamate receptor subunit plays an important role in the process of the vestibnlar compensation. After UL in the knock out mice deficient in delta 2 Glutamate Receptor Subunit, c-fos positive cells are greater in number in the contralateral sides in Mve and SpVe than normal, very few in the contralateral PrH and positive in ipsilesionai IO, contrary to normal mice. We assume that the in the knock-out mice which have dysfunctions in the floccules, the neurons in the vestibular nucleus on the contra latreal side became hyperactive because of the disinhibition from the cerebellum. And in knock out mice lacking control from the cerebellar floccules, c-fos was not activated in PrH on this side.

谷氨酸受体的离子营养性三角洲 - 仅在神经系统中的小脑普kinje细胞中特异性表达，而在三角洲2蛋白中缺乏的突变小鼠已经升高（Kashiwabuchi 1995）。这些小鼠确实表现出长期的抑郁症（LTD），这是一种形式的抑制性（LTD），这是仿制型的（HIRATIC）。我们检查了这些突变小鼠单侧迷路切除术（UL）后检查了LTD在前庭补偿中的参与后，检查了单侧迷宫切除术（UL）后的姿势右反射（VOR）。 And we also examined the c-Fos expression after unilateral ladyrinthectomy in these mutant mice, to investigate the morphological change during the vestibular compensation.After UL in the knock out mice deficient in delta 2 glutamate receptor subunit, the recovery of the postural righting reflex and VOR are retarded than normal mice, which suggests delta 2 glutamate receptor subunit plays an important role in the process of the前卫补偿。在Delta 2谷氨酸受体亚基缺乏的小鼠中UL中，C-FOS阳性细胞在MVE和SPVE的对侧边的数量大于正常PRH，而在Ipsilesionai IO中，与正常小鼠相反。我们假设在絮凝物中具有功能障碍的敲除小鼠中，由于小脑的抑制作用，相反侧的前庭核中的神经元变得过度活跃。在敲出缺乏小脑絮凝物无法控制的小鼠时，在这一侧的PRH中没有激活C-Fos。