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Intracellular Ca2+ mobilization and measurements of nitric oxide synthesis on cultured nasal microvascular endothelial cells

培养的鼻微血管内皮细胞的细胞内 Ca2 动员和一氧化氮合成的测量

基本信息

批准号：
12671689
负责人：
TSUJI Hiroyuki
金额：
$ 2.05万
依托单位：
Kansai Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Besides culture human nasal mucosa vascular endothelial cells, we reviewed the influence of the nitric oxide to the establishment of the culture system of the human nasal mucosa vascular endothelial cells which we obtained at the time of surgery and epithelium ciliary movement, but the establishment of supporting cells is difficult and does not succeed in the measurement of the ciliary movement in the cultured cell.It combines the image analysis device which we purchased newly with electronic flashes copying and reviews the measurement system now.It was under study, and histamin and the leukotoluene load carried out an experiment in the experiment system of the same time measurement of calcium level in cells by fura-2 using the vascular endothelial cells which there was and the quantity of NO production in cells by DAF-2 in last year. As for the elevation of the intracellular calcium level of cultured vascular endothelial cells in the histamine load in our experiment system, it reveale … More d the NO production whereas it was found from the load direct back since it passed after load more than 10 minutes. The concentration-dependency was not clear about the NO production. An approximately similar tendency was present about the leukotoluene.The NO production when we added lipopolysaccharide (LPS) in culture medium beforehand was enhanced, and manifestation of iNOS was suggested..From these examinations, NO of nasal mucosa vascular endothelial cells-producing ability contributed to the microcirculation of the nasal mucosa, and, as for the nasal congestion to be found in allergic rhinitis, it seemed that fall of the NO production ability by the vascular endothelial cells disorder was one of cause. The function of the ciliated epithelium is controlled from a past report by NO, and, as for the NO, that epidermic cells produce is suggested vascular endothelial cells by shearing stress by LPS stimulation. It was thought that this both produced the NO derived from a nasal mucosa excreted by exhalation and determined these mutual relations and connection with the clinical condition in this study. Less

除培养人鼻粘膜血管内皮细胞外，我们还综述了一氧化氮对手术获得的人鼻粘膜血管内皮细胞培养体系的建立及上皮纤毛运动的影响，但是支持细胞的建立是困难的，并且在培养细胞中纤毛运动的测量中不成功。我们新购买的电子闪光复印和评论的测量系统，它是在研究中，组胺和白甲苯负荷进行了实验的实验系统中的同时测量细胞内钙水平的Fura-2使用的血管内皮细胞，其中有和NO的产生量的细胞中的NO-2在去年。在本实验体系中，组胺负荷引起培养的血管内皮细胞内钙离子水平升高，提示组胺负荷可引起血管内皮细胞内钙离子浓度升高， ...更多信息 d的NO产生，而它是从负载直接返回，因为它通过负载后超过10分钟。NO产生的浓度依赖性不明显。在培养液中预先加入脂多糖（LPS）可促进NO的生成，提示有iNOS的存在。根据这些检查，鼻粘膜血管内皮细胞产生NO的能力有助于鼻粘膜的微循环，并且，对于在变应性鼻炎中发现的鼻充血，似乎血管内皮细胞紊乱导致的NO产生能力下降是原因之一。根据以往的报道，纤毛上皮的功能由NO控制，并且，对于NO，表皮细胞产生的建议是血管内皮细胞通过LPS刺激的剪切应力。认为这两者都产生了通过呼气排出的鼻粘膜来源的NO，并确定了这些相互关系和与本研究中的临床状况的联系。少