Study of skin reconstruction by using the technique of tissue engineering

本研究利用纤维蛋白和其他生物材料构建组织工程皮肤。角质形成细胞在纤维蛋白凝胶基质上培养，但角质形成细胞容易分泌蛋白酶消化的纤维蛋白凝胶。因此，作为支架的纤维蛋白凝胶基质的机械强度是不稳定的。当一种可溶性结缔组织材料（专利申请中）混合在纤维蛋白凝胶基质中时，角质形成细胞的蛋白酶消化受到保护。此外，通过使用新型纤维蛋白支架，真皮成纤维细胞可以在凝胶基质中培养，然后角质形成细胞在凝胶基质上培养。这些结果表明，这种新型纤维蛋白支架可能是组织工程皮肤等有用的装置。本研究尝试将新型支架材料应用于组织工程皮肤的临床研究。

In this investigation, tissue engineered skin was designed by using the fibrin and other one. Keratinocytes were cultured onto the fibrin gel matrix but keratinocytes secreted protease digested fibrin gel easily. Therefore, the mechanical strength of fibrin gel matrix as the scaffold was labile. When one soluble connective tissue material (patent pending) was mixed in the fibrin gel matrix, the protease digestion by keratinocytes was protected. Furthermore, by using the new type fibrin scaffold, dermal fibroblasts could be cultured in gel matrix and then keratinocytes onto the gel matirix. From these results, this new type fibrin scaffold might be useful device such as tissue-engineered skin. Now we attempted to apply to this tissue-engineered skin by using new type scaffold clinically.