Study of skin reconstruction by using the technique of tissue engineering

利用组织工程技术重建皮肤的研究

• 批准号: 12671754
• 负责人: KUMAGAI Norio
• 金额: $ 2.43万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671754/
• 关键词: fibrin; keratinocyte; culture; scaffold; tissue engineered epithelium; tissue engineered dermis; tissue engineered skin; 真皮; 再生組織; 培養表皮; 線維芽細胞; ゼラチン

In this investigation, tissue engineered skin was designed by using the fibrin and other one. Keratinocytes were cultured onto the fibrin gel matrix but keratinocytes secreted protease digested fibrin gel easily. Therefore, the mechanical strength of fibrin gel matrix as the scaffold was labile. When one soluble connective tissue material (patent pending) was mixed in the fibrin gel matrix, the protease digestion by keratinocytes was protected. Furthermore, by using the new type fibrin scaffold, dermal fibroblasts could be cultured in gel matrix and then keratinocytes onto the gel matirix. From these results, this new type fibrin scaffold might be useful device such as tissue-engineered skin. Now we attempted to apply to this tissue-engineered skin by using new type scaffold clinically.

本研究利用纤维蛋白和其他材料构建了组织工程皮肤。角质形成细胞被培养到纤维蛋白凝胶基质上，但角质形成细胞分泌的蛋白酶容易消化纤维蛋白凝胶。因此，纤维蛋白凝胶基质作为支架材料的机械强度不稳定。当一种可溶性结缔组织材料(正在申请专利)混合在纤维蛋白凝胶基质中时，角质形成细胞的蛋白酶消化得到保护。此外，利用新型纤维蛋白支架，真皮成纤维细胞可以在凝胶基质中培养，然后角质形成细胞可以在凝胶基质上生长。从这些结果来看，这种新型的纤维蛋白支架可能是一种有用的装置，如组织工程皮肤。现在，我们尝试将新型支架应用于这种组织工程皮肤。