Study of skin reconstruction by using the technique of tissue engineering

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In this investigation, tissue engineered skin was designed by using the fibrin and other one. Keratinocytes were cultured onto the fibrin gel matrix but keratinocytes secreted protease digested fibrin gel easily. Therefore, the mechanical strength of fibrin gel matrix as the scaffold was labile. When one soluble connective tissue material (patent pending) was mixed in the fibrin gel matrix, the protease digestion by keratinocytes was protected. Furthermore, by using the new type fibrin scaffold, dermal fibroblasts could be cultured in gel matrix and then keratinocytes onto the gel matirix. From these results, this new type fibrin scaffold might be useful device such as tissue-engineered skin. Now we attempted to apply to this tissue-engineered skin by using new type scaffold clinically.