Study of skin reconstruction by using technique of regenerative medicine

利用再生医学技术重建皮肤的研究

• 批准号: 17591879
• 负责人: KUMAGAI Norio
• 金额: $ 2.18万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591879/
• 关键词: cultured epithelium; epithelial stem cells; cell therapy; skin regeneration

[Objective] To evaluate the stem cells therapy for refractory skin ulcer, we attempted to graft the ulcer region cultured epithelial stem cells.[Methods] The cell suspension of epithelial stem cells was applied on severe pressure ulcers with deep subcutaneous undermining ; i.e. cultured epithelial stem cells that did not reach until the confluence were harvested by using ordinary method after EDTA treatment for removing 3T3 feeder layer. The cultured epithelial stem cell, which was adjusted cell number, was applied undermining ulcer region with bio-gel (pat. pending). The epithelial sheet, which was cultured until confluent, was applied on the ordinary refractory skin ulcer. After the infection of ulcer wound was completely regulated by using various disinfectants, the cell therapy was performed. The epithelial stem cells applied on wound surface were dressed according to ordinary methods. The cell therapy was attempted once per a week during 4 weeks as far as circumstances permit.[Results] All of these patients, who resisted against the ordinary therapy, obtained significantly reduction of wound area or reepithelization. The shortest period of the therapy was 2 times of treatment during 2 weeks but many patients reepithelized within 40 days by using this method.[Discussion] At present, moist wound healing devise or cytokine such as FGF-2 are used for refractory skin ulcer. However, the wound healing process, which consist of the complicated mechanism, is not regulated by just one cytokine. Therefore, in case of these refractory skin ulcer patients, it was suggested that the release of various cytokines in epithelial stem cells ; i.e., good balance and coordination of cytokine network, was important such as the effective mechanism of cell therapy

[目的]为评价干细胞治疗难治性皮肤溃疡的效果，将培养的皮肤上皮干细胞移植到溃疡区。[方法]将上皮干细胞悬液应用于深度皮下破坏的重度压疮，即在EDTA处理去除3T3饲养层后，用普通方法采集未达融合的培养上皮干细胞。将培养的上皮干细胞经细胞数调整后，应用生物凝胶(PAT)破坏溃疡区。待定)。将培养至融合的上皮片应用于普通难治性皮肤溃疡。在各种消毒剂完全控制溃疡创面感染后，进行细胞治疗。应用于创面的上皮干细胞按常规方法包扎。在条件允许的情况下，每周进行一次细胞治疗。[结果]所有抗拒常规治疗的患者创面面积均明显缩小或再上皮化。最短的疗程是2周内治疗2次，但许多患者在40天内重新上皮化。[讨论]目前，难治性皮肤溃疡多采用湿润创面愈合仪或细胞因子如成纤维细胞生长因子-2等。然而，创伤愈合过程由复杂的机制组成，并不是由单一的细胞因子调控的。因此，对于这些难治性皮肤溃疡患者，人们认为上皮干细胞释放各种细胞因子，即细胞因子网络的良好平衡和协调，是细胞治疗的有效机制。

项目成果

多血小板血漿を用いた新規ヒト細胞培養法の確立 -自家・他家血小板血漿利用による培養表皮作成とその可能性-

使用富含血小板的血浆建立新的人体细胞培养方法 - 使用自体和同种异体血小板血浆创建培养表皮及其可能性 -

• 发表时间: 2006
• 期刊: 聖マリアンナ医科大学雑誌 34・4
• 作者: Ichiro;Tanaka;Ichiro Tanaka;中野 江里子 他
• 通讯作者: 中野 江里子 他

エンドセリン-1拮抗薬FR139317が皮弁内6keto-PGF_<1α>/TX-B_2動態に及ぼす影響

内皮素-1拮抗剂FR139317对皮内皮瓣6酮-PGF_<1α>/TX-B_2动力学的影响

• 发表时间: 2006
• 期刊: 聖マリアンナ医科大学雑誌 34・4
• 作者: Ichiro;Tanaka;Ichiro Tanaka;中野 江里子 他;門司 明夫 他
• 通讯作者: 門司 明夫 他

培養表皮細胞からのVEGF産生に及ぼすエンドセリンの影響

内皮素对培养表皮细胞产生 VEGF 的影响

• 发表时间: 2006
• 期刊: 聖マリアンナ医科大学雑誌 34・4
• 作者: Yoshiro Hayashi;et. al.;中野 江里子 他;門司 明夫 他;安藤(鹿井) 史子 他
• 通讯作者: 安藤(鹿井) 史子 他

Influence on PGF1 α/TXB2 Ratio with Endothelin-1 Production in Flap

PGF1α/TXB2 比值对皮瓣内皮素-1 产生的影响

• 发表时间: 2006
• 期刊: J. St. Marianna Univ. Sch. Med. 34(4)
• 作者: Ichiro;Tanaka;Ichiro Tanaka;中野 江里子 他;門司 明夫 他;安藤(鹿井) 史子 他;Fumiko A et al.;Akio M et al.
• 通讯作者: Akio M et al.

The Influence of Endothelins on VEGF Production in Normal Human Cultured Keratinocytes

内皮素对正常人培养角质形成细胞中 VEGF 产生的影响

• 发表时间: 2006
• 期刊: J. St. Marianna Univ. Sch. Med. 34(4)
• 作者: Ichiro;Tanaka;Ichiro Tanaka;中野 江里子 他;門司 明夫 他;安藤(鹿井) 史子 他;Fumiko A et al.
• 通讯作者: Fumiko A et al.