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FUNCTION OF DESCENDING CONTROL AND NEUROACTIVE SUBSTANCE RECEPTORS IN OROFACIAL PAIN

下降控制和神经活性物质受体在口面部疼痛中的功能

基本信息

批准号：
12671798
负责人：
HAYASHI Haruhide
金额：
$ 2.43万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671798/
关键词：
NK-1 receptor nociceptine neuron C-fiber response pain raphe magnus descending inhibition spinal dorsal horn nociception 慢性炎症性疼痛動物 α_2受容体 Yohimbine

项目摘要

NK-1 receptor antagonist inhibited the late discharges evoked by C-fiber input in the spinal nociceptive neurons, suggesting the role of substance P in acute pain. The antagonist, however, failed to inhibit them in the majority of spinothalamic neurons. This experiment was aimed to investigate the role of NK-1 receptor in the spinal interneurons during the descending inhibition via the nucleus raphe magnus (NRM).Under pentobarbital sodium anesthesia (50 mg/kg) and immobilization with pancronium bromide, single unit recordings were made from the nociceptive specific or wide dynamic range neurons in the lamina I of lumber dorsal horn of Wistar rats. They were tested for projections to C_3 or higher levels of brain and the late discharges (100-400 ms) evoked by electrical stimulations of the receptive fields and the effects of NRM electrical stimulations (a train of 20 pulses, 0.2 ms duration, 40 Hz). After physiological characterization, a dose of 18-37 nmol of RP-67580 (rat NK-1 receptor antagonist) was applied onto the cord surface and washed out after 10-20 min. The C-discharges were counted every 10-15 min for 60-90 min.Sixty three neurons were recorded and 43 interneurons were analyzed. A significant relationship was found between the magnitudes of maximum inhibitions by the antagonist and NRM stimulation (correlation 0.73).

NK-1受体拮抗剂可抑制脊髓痛觉神经元c -纤维输入引起的晚期放电，提示P物质在急性疼痛中的作用。然而，拮抗剂在大多数脊髓丘脑神经元中未能抑制它们。本实验旨在探讨NK-1受体在中隔大核下行抑制过程中在脊髓中间神经元中的作用。在戊巴比妥钠麻醉（50 mg/kg）和溴化戊溴铵固定下，对Wistar大鼠腰背角I层痛觉特异性或宽动态范围神经元进行单单位记录。他们被测试到C_3或更高水平的大脑和后期放电（100-400毫秒）由电刺激的感受野和NRM电刺激的影响（20脉冲，0.2毫秒持续时间，40赫兹）。生理鉴定后，将剂量为18-37 nmol的RP-67580（大鼠NK-1受体拮抗剂）涂于脐带表面，10-20 min后冲洗。每10-15分钟计数一次c -放电，持续60-90分钟。记录63个神经元，分析43个中间神经元。拮抗剂的最大抑制强度与NRM刺激之间存在显著相关（相关系数0.73）。