Exhaustive analysis of gene expression dining bone regeneration

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We conducted the following; experiments.1. Characterizaiion of mecenchymal stem cells during bone regenerationWe first made n round hole, 2.2 mm in a diameter, at diaphysis of femurs of growing mice To Investigate the proliferation activity of mesenchymal cells during the bone repair, we explored incorporation of BrdU. These studies demonstrated that proliferating mesenchymal cells incorporating BrdU increased 3 days after injury in our experimental system.2. Changes in gene expression during bone regenerationUsing above described experimental model, we investigated the changes in expression patterns of bone-related genes by Northern blot analysis. ALP mRNA increased on day 4 and osteocalcin mRNA increased OB day 5 after bone injury. Histochemical and immunohistochemical studies indicated similar expression patterns of these molecules.3. Above experiments suggested that mesenchymal stem cells appeared during days 4 and 5 after injury-in our experimental model. We are now exploring the genes related to mesenchymal stem cells by cDNA chips.